Summary
Sinusoidal length deformations were applied to bundles of one to three fibres from glycerol-extractedLethocerus flight muscle under conditions of high calcium activation (pCa=6) and in the absence of phosphate. The mechanical response was found to be significantly non-linear under these conditions for amplitudes of the order of 0.05% and greater. The non-linearity was analysed into harmonic components by Fourier analysis, data for this being collected and stored in a computer. The largest harmonic components were found to be the first and second; these components were particularly prominent at frequencies close to those producing maximum phase shift between length and fundamental tension amplitude. At very low amplitudes (<0.02%) the non-linearities became very small and could not be readily detected. At this very low amplitude the fundamental could be considered as the linear response to the length change; it had a much sharper frequency dependence than previously recorded fromLethocerus flight muscle. The variation of the response with imposed conditions has been studied.
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References
ABBOTT, R. H. (1969) D. Phil. thesis, Oxford University.
ABBOTT, R. H. (1973) The effect of fibre length and calcium ion concentration on the dynamic response of glycerol extracted insect fibrillar muscle.J. Physiol. 231, 195–208.
JEWELL, B. R., PRINGLE, J. W. S. & RÜEGG, J. C. (1964) Oscillatory contraction of insect fibrillar muscle after glycerol extraction.J. Physiol. 173, 6–8.
JEWELL, B. R. & RÜEGG, J. C. (1966) Oscillatory contraction of insect fibrillar muscle after glycerol extraction.Proc. R. Soc. 164, 428–59.
MACHIN, K. E. & PRINGLE, J. W. S. (1960) The physiology of insect fibrillar muscle. III. The effect of sinusoidal length changes on a beetle flight muscle.Proc. R. Soc. 152, 311–30.
PERRIN, D. D. & SAYCE, I. G. (1967) Computer calculations of equilibrium concentrations in mixtures of metal ions and complexing species.Talanta 14, 833–42.
PRINGLE, J. W. S. & TREGEAR, R. T. (1969) Mechanical properties of insect fibrillar muscle at large amplitudes of oscillation.Proc. R. Soc. 174, 33–50.
SILLEN, L. G. & MARTELL, A. E. (1964) Stability constants of metal-ion complexes.Chem. Soc. Spectrosc. Publn. 17.
STEIGER, C. J. (1977) Stretch activation and tension transients in cardiac skeletal and insect flight muscle. InInsect Flight Muscle (edited by TREGEAR, R. T.), pp. 221–68. Amsterdam: North Holland.
THORSON, J. & WHITE, D. C. S. (1969) Distributed representations for actin-myosin interaction in the oscillatory contraction of muscle.Biophys. J. 9, 360–90.
WHITE, D. C. S. & THORSON, J. (1972) Phosphate starvation and the nonlinear dynamics of insect fibrillar flight muscle.J. gen. Physiol. 60, 307–36.
WILSON, M. G. A., WHITE, D. C. S. & THORSON, J. (1979) What do the mechanics of relaxed insect flight muscle tell us about the mechanism of active contraction? InCross-bridge Mechanism in Muscle Contraction (edited by SUGI, H. and POLLACK, G. H.), pp. 193–209. Baltimore: University Park Press.
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Cuminetti, R., Rossmanith, G. Small amplitude non-linearities in the mechanical response of an asynchronous flight muscle. J Muscle Res Cell Motil 1, 345–356 (1980). https://doi.org/10.1007/BF00711935
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DOI: https://doi.org/10.1007/BF00711935