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Genetics of alkane oxidation byPseudomonas oleovorans

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Abstract

Many Pseudomonads are able to use linear alkanes as sole carbon and energy source. The genetics and enzymology of alkane metabolism have been investigated in depth forPseudomonas oleovorans, which is able to oxidize C5-C12 n-alkanes by virtue of two gene regions, localized on the OCT-plasmid. The so-calledalk-genes have been cloned in pLAFR1, and were subsequent analyzed using minicell expression experiments, DNA sequencing and deletion analysis. This has led to the identification and characterization of thealkBFGHJKL andalkST genes which encode all proteins necessary to convert alkanes to the corresponding acyl-CoA derivatives. These then enter the β-oxidation-cycle, and can be utilized as carbon- and energy sources. Medium (C6-C12)- or long-chain (C13-C20) n-alkanes can be utilized by many strains, some of which have been partially characterized. The alkane-oxidizing enzymes used by some of these strains (e.g. twoP. aeruginosa strains, aP. denitrificans strain and a marinePseudomonas sp.) appear to be closely related to those encoded by the OCT-plasmid.

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  1. Institute of Biotechnology, ETH-Hönggerberg, 8093, Zürich, Switzerland

    Jan B. van Beilen, Marcel G. Wubbolts & Bernard Witholt

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van Beilen, J.B., Wubbolts, M.G. & Witholt, B. Genetics of alkane oxidation byPseudomonas oleovorans . Biodegradation 5, 161–174 (1994). https://doi.org/10.1007/BF00696457

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