Abstract
Liquid cell suspension cultures derived from marine plants have the potential to biosynthesize novel biomedicinal compounds in a controlled environment. Of particular interest are the eicosanoids and related oxylipins emanating from the 15-lipoxygenase manifold of the arachidonic acid cascade, which is active in the brown algaLaminaria saccharina. Filamentous cell clumps ofL. saccharina isolated from female gametophytes were cultured in an illuminated bubble-column bioreactor in GP2 artificial seawater nutrient medium at 13 °C and air flow rate of 0.35 L air min−1 L−1 culture (vvm). Growth kinetics and biomass productivity data were obtained as a function of incident light intensity (2.4 to 98μmol photon m−2 s−1) and initial cell density (27 to 149 mg DCW L−1). Maximum cell densities exceeded 1200 mg DCW L−1 after a 20 day cultivation time at optimal conditions of 98μmol photon m−2 s−1 and 118 mg DCW L−1 initial cell density. Qualitative analysis of chloroform/methanol extracts of the cell culture biomass by GC-MS confirmed the presence of the hydroxy fatty acids 13-HODTA and 13-HOTE, the likely products of 15-lipoxygenase catalyzed oxidation of linoleic or linolenic acids.
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References
Bradley PM, Cheney DP (1990) Some effects of plant growth regulators on tissue cultures of the marine red algaAgardhiella subulata (Gigartinales, Rhodophyta). Hydrobiologia 204/205 (Dev. Hydrobiol. 58): 353–60.
Butler DM, Evans LV (1990) Cell and tissue culture of macroalgae. In: Akatsuka I (ed.), Introduction to Applied Phycology. SPB Academic Publishing by, The Hague, Netherlands: 629–645.
Chen LCM (1989) Cell suspension culture fromPorphyra linearis (Rhodophyta) a multicellular marine red alga. J. appl. Phycol. 1: 153–159.
Chen LCM (1990) Biotechnology potential for Hoshi-Nori production. World Aquaculture 21: 106–107.
Cheney DP (1986) Genetic engineering in seaweeds: Applications and current status. Beifhefte zur Nova Hedwigia 83: 22–29.
Cheney DP, Luistro AH, Bradley PM (1987) Carrageenan analysis of tissue cultures and whole plants ofAgardhiella subulata. Hydrobiologia 151/152 (Dev. Hydrobiol. 41): 161–166.
Fusetani N, Hashimoto K (1984) Prostaglandin E2: A candidate for causative agent of ‘Ogonori’ poisoning. Bull. Jpn. Soc. Sci. Fish. 50: 465–469.
Gerwick WH, Bernart MW (1993) Eicosanoids and related compounds from marine algae. In: Zaborski OR, Attaway DH (eds), Marine Biotechnology, Volume 1: Pharmaceutical and Bioactive Natural Products. Plenum, NY: 101–150.
Gerwick WH, Bernart MW, Moghaddam MF, Jiang ZD, Solem ML, Nagle DG (1990) Eicosanoids from the Rhodophyta: new metabolism in the algae. Hydrobiologia 204/205 (Dev. Hydrobiol. 58): 621–628.
Gerwick WH, Protean PJ, Nagle DN, Wise ML, Jiang ZD, Bernart MW, Hamberg M (1993) Biologically active oxylipins from seaweeds. Hydrobiologia 260/261 (Dev. Hydrobiol. 58): 653–665.
Gregson RP, Marwood JF, Quinn RJ (1979) The occurrence of prostaglandins PGE2 and PGF2α . in a plant — The red algaGracilaria lichenoides. Tetrahedron Lett. 20: 4505–4506.
Jiang ZD, Gerwick WH (1991) Eicosanoids and other hydroxylated fatty acids from the marine algaGracilariopsis lemaneiformis. Phytochemistry 30: 1187–1190.
Kurata K, Taniguchi K, Shiraishi K, Hayama N, Tanaka I, Suzuki M (1989) Ecklonialactone-A and -B, two unusual metabolites from the brown algaEcklonia stolonifera Okamura. Chem. Lett. 1989: 267–270.
Liu XW, Rochas C, Kloareg B (1990) Callus culture ofPterocladia capillacea (Rhodophyta) and analysis of cell wall polysaccharides. J. appl. Phycol. 2: 297–303.
Long, W., Staples J., Tait M., Williamson F., Wilson F (1989) Axenic cell culture of Rhodophytes for specialty polysaccharide production. In: Kain JM, Andrews JW, McGregor BJ (eds), COST 48 Conference Proceedings, Commission of the European Communities, Brussels: 51–54.
Lüning K (1980) Critical levels of light and temperature regulating the gametogenesis of three Laminaria species (Phaeophyceae). J. Phycol. 16: 1–15.
Lüning K, Dring MJ (1972) Reproduction induced by blue light in female gametophytes ofLaminaria saccharina. Planta 104: 252–256.
Lüning K, Dring MJ (1975) Reproduction, growth, and photosynthesis of gametophytes ofLaminaria saccharina grown in blue and red light. Mar. Biol. 29: 195–200.
Lüning K, Neushul M (1978) Light and temperature demands for growth and reproduction of Laminarian gametophytes in southern and central California. Mar. Biol. 45: 297–309.
Moghaddam MF, Gerwick WH, Ballantine DL (1990) Discovery of the mammalian insulin release modulator, Hepoxilin B3, from the tropical red algaePlatysiphonia miniata andCottoniella filamentosa. J. biol. Chem. 265: 6126–6130.
Montomura T, Sakai Y (1984) Regulation of gametogenesis of Laminaria and Desmarestia (Phaeophyta) by iron and boron. Jap. J. Phycol. 32: 209–215.
Nelson NA, Kelley RC, Johnson RA (1982) Prostaglandins and the arachidonic acid cascade. C&EN 60(30): 30–44.
Peimin HE, Wang S (1992). Cell suspension culture in Porphyra. J. Phycol. 28: 7.
Polne-Fuller M, Saga N, Gibor A (1986). Algal cell, callus and tissue cultures and selection of algal strains. Beihefte zur Nova Hedwigia 83: 30–36.
Polne-Fuller M, Gibor A (1987) Calluses and callus-like growth in seaweeds: Induction and culture. Hydrobiologia 151/152 (Dev. Hydrobiol. 41): 131–138.
Polne-Fuller M (1988) The past, present, and future of tissue culture and biotechnology of seaweeds In: Stadler T, Mollion J, Verdis MC, Karamanos Y, Horvan D, Christiaen D (eds), Algal Biotechnology. Elsevier Applied Science: 17–31.
Polne-Fuller M, Gibor A (1990) Developmental studies in Porphyra (Rhodophyceae) III. Effect of culture conditions on wall regeneration and differentiation of protoplasts. J. Phycol. 26: 674–682.
Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophyllsa andb extracted with four different solvents. Biochem. Biophys. Acta 975: 384–394.
Proteau PJ, Gerwick WH (1992) Cymathere ethers A and B: Biocyclic oxylipins from the brown algaCymathere triplicata. Tetrahedron Lett. 33: 4393–4396.
Proteau N, Gerwick WH (1993) Divinyl ethers and hydroxy fatty acids from three species ofLaminaria (Brown algae). Lipids 28: 783–787.
Steele RL, Thursby GB (1988) Laboratory culture of gametophytic stages of the marine macroalgaeChampia parvula (Rhodophyta) andLaminaria saccharina (Phaeophtya). Environ. Tox. Chem. 7: 997–1002.
Tait MI, Milne AM, Grant D, Somer JA, Staples J, Long WF, Williamson FB, Wilson SB (1990) Porphyra cell cultures: isolation, growth and polysaccharide production. J. appl. Phycol. 2: 63–70.
Wasserman MA, Smith EF, Underwood DC, Barnette MA (1991) Pharmacology and pathology of lipoxygenase products. In: Cooke ST, Wong A (eds), Lipoxygenases and their Products. Academic Press: 1–50.
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Rorrer, G.L., Modrell, J., Zhi, C. et al. Bioreactor seaweed cell culture for production of bioactive oxylipins. J Appl Phycol 7, 187–198 (1995). https://doi.org/10.1007/BF00693067
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DOI: https://doi.org/10.1007/BF00693067