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Experimental allergic myositis: strain 13 guinea pig immunised with rabbit myosin B fraction

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Summary

Of 32 guinea pigs (Hartley and strain 13), 20 were immunised with whole muscle homogenate, microsome or myosin B fraction of rabbit skeletal muscle with Freund's complete adjuvant (FCA); the purified myosin fraction was also used as an antigen in four animals at a concentration lower than other antigens; four were injected with normal saline and FCA; other four had no injection. Five histological changes, namely necrosis, phagocytosis, central nuclei, inflammation and vacuoles, in the quadriceps femoris muscle were compared by quantitative analysis. The group immunised with myosin B fraction showed necrosis, phagocytosis and inflammation more frequently than those immunised with other preparations (p<0.05). The changes were more frequent in the strain 13 than in Hartley (p<0.05).

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References

  1. Dawkins RL (1965) Experimental myositis associated with hypersensitivity to muscle. J Pathol Bacteriol 90:619–625

    Google Scholar 

  2. Dubowitz V, Brooke MH (1973) Muscle biopsy. A modern approach. Saunders, London, pp 231–241

    Google Scholar 

  3. Dunkin GW, Hartley P, Lewis-Faning E, Russell WT (1930) A comparative biometric study of albino and coloured guinea-pigs from the point of view of their suitability for experimental use. J Hyg (Lond) 30:311–330

    Google Scholar 

  4. Ebashi S, Lipmann F (1962) Adenosine triphosphate-linked concentration of calcium ions in a particulate fraction of rabbit muscle. J Cell Biol 14:389–400

    Google Scholar 

  5. Geczy AF, de Weck AL, Schwartz BD, Shevach EM (1975) The major histocompatibility complex of the guinea pig. I. Serologic and genetic studies. J Immunol 115:1704–1710

    Google Scholar 

  6. Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177:751–766

    Google Scholar 

  7. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  8. Manghani D, Partridge TA, Sloper JC (1974) The role of the myofibrillar fraction of skeletal muscle in the production of experimental polymyositis. J Neurol Sci 23:489–503

    Google Scholar 

  9. O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    Google Scholar 

  10. Ogawa Y (1970) Some properties of fragmented frog sarcoplasmic reticulum with particular reference to its response to caffeine. J Biochem (Tokyo) 67:667–683

    Google Scholar 

  11. Perry SV (1955) Myosin adenosinetriphosphatase. Methods Enzymol 2:582–589

    Google Scholar 

  12. Richards EG, Chung C-S, Menzel DB, Olcott HS (1967) Chromatography of myosin on diethylaminoethylsephadex A-50. Biochemistry 6:528–540

    Google Scholar 

  13. Rosenberg NL, Ringel SP, Kotzin BL (1985) Experimental autoimmune myositis in SJL/J mice. Ann Neurol 18:161

    Google Scholar 

  14. Rosenthal AS, Shevach EM (1973) Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med 138:1194–1212

    Google Scholar 

  15. Szent-Györgyi A (1951) Chemistry of muscle contraction, 2nd edn. Academic Press, New York, pp 151–152

    Google Scholar 

  16. Tal C, Liban E (1962) Experimental production of muscular dystrophy-like lesions in rabbits and guinea-pigs by an autoimmune process. Br J Exp Pathol 43:525–529

    Google Scholar 

  17. Webb JN (1970) Experimental immune myositis in guinea pigs. Res J Reticuloendothel Soc 7:305–316

    Google Scholar 

  18. Weber K, Osborn M (1969) The reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide gel electrophoresis. J Biol Chem 244:4406–4412

    Google Scholar 

  19. Whitaker JN (1982) Inflammatory myopathy. A review of etiologic and pathogenetic factors. Muscle Nerve 5:573–592

    Google Scholar 

  20. Wisniewski HM, Kieth AB (1977) Chronic relapsing experimental allergic encephalomyelitis. An experimental model of multiple sclerosis. Ann Neurol 1:144–148

    Google Scholar 

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Authors and Affiliations

  1. Department of Neurology, Kanazawa University School of Medicine, 13-1 Takaramachi, 920, Kanazawa City, Japan

    S. Matsubara & M. Takamori

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  1. S. Matsubara
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  2. M. Takamori
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Supported in part by a grant from the Neuroimmunological Disease Research Committee (Japanese Ministry of Health and Welfare) and by Aid for Scientific research (Japanese Ministry of Education). This paper was read to the 13th World Congress of Neurology, Hamburg, 1985

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Matsubara, S., Takamori, M. Experimental allergic myositis: strain 13 guinea pig immunised with rabbit myosin B fraction. Acta Neuropathol 74, 158–162 (1987). https://doi.org/10.1007/BF00692846

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  • DOI: https://doi.org/10.1007/BF00692846

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