Summary
All classes of non-immune mouse immunoglobulins bind non-specifically to the cytoplasm of modified astroglial cells, swollen-bodied astrocytes, when applied to paraffin or frozen sections of human brain tumors and various reactive states. The binding could be inhibited by preincubation with normal human serum, and a proportion of the cells, which reacted with mouse immunoglobulins, showed spontaneous presence of various classes of human immunoglobulins. The Fc part of the immunoglobulins was involved in the binding, since preincubation of mouse immunoglobulins with protein A effectively inhibited the binding and the F(ab)2 fragments of mouse immunoglobulins showed no affinity to modified astroglial cells. The nonspecific binding reported here effectively precludes the use of whole mouse immunoglobulins in the immunohistochemistry of human brain pathology.
Similar content being viewed by others
References
Brett M, Weller RO (1978) Intracellular serum proteins in cerebral gliomas and metastatic tumors: an immunoperoxidase study. Neuropathol Appl Neurobiol 4:263–272
Caine GD, Weller RO, Davies BE, Cox S (1980) Mechanism of uptake and the fate of serum proteins and horseradish peroxidase in cultured human glioma cells. A light and electron immunocytochemical study. Acta Neuropathol (Berl) 52:169–177
DeLellìs RA, Sternberger LA, Mann RB, Banks PM, Nakane PK (1979) Immunoperoxidase technics in diagnostic pathology. Am J Clin Pathol 71:483–488
Esiri M (1977) Immunoglobulin containing cells in multiple sclerosis. Lancet 2:478–480
Hirano A, Matsui T (1975) Vascular structures in brain tumors. Hum Pathol 6:611–621
Hoshino T, Wilson CB, Ellis WG (1975) Gemistocytic astrocytes in gliomas: An autoradiographic study. J Neuropathol Exp Neurol 34:263–281
Hsu S-M, Raine L, Fanger H (1981) Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques. A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Hürter T, Bröcker W, Hossman K-A (1981) Evaluation of vasogenic edema in experimental brain tumors by cathodoluminiscence and fluorescence microscopy. Histochemistry 72:249–254
Nathaniel EJH, Nathaniel DR (1977) Astroglial response to degeneration of dorsal root fibres in adult rat spinal cord. Exp Neurol 54:60–76
Sternberger LA (1978) Immunocytochemistry, 2nd edn. Wiley and Sons, New York
Author information
Authors and Affiliations
Additional information
Supported by grant no. RO 1 CA 23809 from the National Cancer Institute, NIH, Bethesda, MD, USA
Rights and permissions
About this article
Cite this article
Paasivuo, R., Saksela, E. Non-specific binding of mouse immunoglobulins by swollen-bodied astrocytes — a potential source of confusion in human brain immunohistochemistry. Acta Neuropathol 59, 103–108 (1983). https://doi.org/10.1007/BF00691594
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00691594