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Effects of 17-α-methultestosterone and 17-β-oestradiol on intestinal transport and absorption ofl-(14C)-leucine in vitro in rainbow trout (Salmo gairdneri)

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Summary

The effects of 17-α-methyltesterone (MT) and 17-β-oestradiol (E2) on transport and absorption ofl-(14C)-leucine, were studiedin vitro using everted gut sacs of rainbow trout (Salmo gairdneri), incubated at 14°C for 60 min. Hormone administration was carried out either by intramuscular injection (200 μg/100 g·day) on alternate days for 10 days, or by direct addition (200 μg/ml) to incubation media containing sacs from untreated fish (MT+; E2+). Addition of MT to incubation media significantly increased the serosal leucine increase (SLI) and mucosal absorption after 20 min of incubation (Table 1), but did not influence the overall ability of the intestine to accumulate radioleucine (Fig. 1). By contrast, MT injection elicited significant increases in overall intestinal accumulation of radioleucine (Fig. 2) and SLI, but was without effect on mucosal absorption (Table 2). Both MT+ and MT injection resulted in regional variations of radioleucine accumulation along the intestine, being higher in the midgut of the MT+ group (Fig. 1), and higher in the hindgut of the MT injected group (Fig. 2). Injection of E2 significantly increased midgut accumulation (Fig. 2) and SLI, but was without effect on mucosal absorption (Table 2). Addition of E2 into the incubation media however, was without effect on any of the parameters studied, nor were regional variations of radioleucine accumulation detected in any group receiving E2 treatment.

These results suggest that steroid-induced growth promotion in fish may be partly a consequence of enhanced efficiency of intestinal function.

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Abbreviations

MT :

17-α-methyltestosterone

E2 :

17-β-gestradiol

MT+MT :

added to incubation media

E2+E2:

added to incubation media

SLI :

serosal leucine increase

S/M :

serosal/mucosar ratio

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Habibi, H., Ince, B.W. & Matty, A.J. Effects of 17-α-methultestosterone and 17-β-oestradiol on intestinal transport and absorption ofl-(14C)-leucine in vitro in rainbow trout (Salmo gairdneri). J Comp Physiol B 151, 247–252 (1983). https://doi.org/10.1007/BF00689925

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