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Ultrastructural and ionic studies in global ischemic dog brain

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Summary

A time course of tissue ionic changes, and their relation to ultrastructural findings during reperfusion following a 15-min global ischemic brain insult was studied in a dog model. Parietal cortex was analyzed for Ca, Na, K, Mg and Fe in controls and after 10 min, 2, 4, and 8 h of reperfusion. After 8 h of reperfusion, the mean values (μmol/g tissue wet wt.) for Ca (control=1.43, 8 h=2.76) and Na (control 60.4, 8 h=107.4) doubled and K (control=90.4, 8 h=48.5) decreased to half that of the control. Ultrastructural studies and subcellular localization of calcium in parietal cortex of in situ-fixed brains after 8 h showed cortical neurons with clumping of nuclear chromatin, dilatation of endoplasmic reticulum and disruption of plasma membranes. Large amounts of electron-dense precipitates of calcium were present within dilated astrocytic processes, synaptic vesicles, cytoplasm of edematous dendrites and mitochondria. Cortical neurons from postischemic dogs without reperfusion showed only slight chromatin clumping and edema of astrocytic processes, but no calcium accumulation. The large ionic shifts noted between 4 and 8 h of reperfusion, indicate a progressive inability of the cells to maintain normal transmembrane gradients of these ions and may reflect a membrane destructive process, as demonstrated ultrastructurally at 8 h. Enhanced calcium entry into the neuron during reperfusion appears to be a part of the cytotoxic mechanism leading to neuronal necrosis.

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Authors and Affiliations

  1. Department of Pathology, College of Human Medicine, Michigan State University, 48824-1316, East Lansing, MI, USA

    K. Kumar, R. Estrada & A. Koestner

  2. Department of Emergency Medicine, College of Human Medicine, Michigan State University, 48824-1316, East Lansing, MI, USA

    G. S. Krause, T. J. Hoehner & B. C. White

  3. Department of Biochemistry, College of Human Medicine, Michigan State University, 48824-1316, East Lansing, MI, USA

    N. R. Nayini

  4. Department of Emergency Medicine, Butterworth Hospital, Grand Rapids, Michigan, USA

    M. Goosmann

Authors
  1. K. Kumar
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  2. M. Goosmann
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  3. G. S. Krause
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  4. N. R. Nayini
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  5. R. Estrada
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  6. T. J. Hoehner
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  7. B. C. White
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  8. A. Koestner
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Additional information

Supported by Contract No DAMD 85-17-4200 from the United States Army Medical Research and Development Command and a grant from the College of Human Medicine, Michigan State University. This work was presented at the 70th Annual Meeting of the American Association of Pathology, St. Louis, MO, April 16, 1986

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Kumar, K., Goosmann, M., Krause, G.S. et al. Ultrastructural and ionic studies in global ischemic dog brain. Acta Neuropathol 73, 393–399 (1987). https://doi.org/10.1007/BF00688266

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  • DOI: https://doi.org/10.1007/BF00688266

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