Abstract
The full-length cDNA corresponding to the mRNA of the fusion (F) protein of the Yamagata-1 strain of subacute sclerosing panencephalitis (SSPE) virus was cloned, and its complete nucleotide sequence was determined. The F gene was composed of 2369 nucleotides and contained a single large coding region, which is located between two noncoding regions. The 5′-terminal noncoding region consisted of 584 nucleotides comprising 44.9% cytosine, and had several inverted repetitious sequences. The 3′-terminal noncoding region had a relatively low homology of 91.7% with the MV. The coding region was expanded for nucleotides 585–2189, which encoded 534 amino acids with a molecular weight of 57,963. The homology of the amino acid sequence of the F protein between the MV and SSPE virus was 96.27%, and the positions of cysteine and proline were almost identical in the two viruses. The functional domains of SSPE-virus F protein closely resembled those of MV F protein, including the cleavage site, a signal sequence, the fusion-related stretch, the transmembrane region, and four potential glycosylation sites. Four antigenic epitopes on the MV F protein were also conserved on the SSPE-virus F protein.
However, deletion of one nucleotide (position 2155) of the SSPE virus was found when compared with the MV, and shifted the coding frame, causing the substitutions of 27 C-terminal amino acids of the MV F protein with 11 different residues. The variations of the C-terminal region of the F protein were observed with two other SSPE viruses, suggesting that this may be a common property of SSPE virus that differs from MV.
Similar content being viewed by others
References
HommaM. and OhuchiM., J Virol12, 1457–1467, 1973.
ScheidA. and ChoppinP.W., Virology80, 54–66, 1977.
HardwickJ.M. and BussellR.H., J Virol25, 687–692, 1978.
RichardsonC.D., ScheidA. and ChoppinP.W., Virology105, 205–222, 1980.
RichardsonC. and ChoppinP.W., Virology131, 518–532, 1983.
NorrbyE., ChenS-N., TogashiT., ShesberadaranH. and JohnsonK.P., Arch Virol71, 1–11, 1982.
SatoT.A., FukudaA. and SugiuraA., J Gen Virol66, 1397–1409, 1985.
DoiY., SanpeT., NakajimaM., OkawaS., KatohT., ItohH., SatoT., OguchiK., KumanishiT. and TsubakiT., Jpn J Med Sci Biol25, 321–333, 1972.
JohnsonK.P., NorrbyE., SwovelandP. and CarriganD.R., Arch Virol,73, 255–262, 1982.
UedaS., OkunoY., OkunoY., HamamotoY. and OhyaH., Biken's J18, 113–122, 1975.
RichardsonC., HullD., GreerP., HaselK., BerkovichA., EnglundG., BelliniW., RimaB. and LazzariniR., Virology155, 508–523, 1986.
BucklandR., GeraldC., BarkerR. and WildT.F., J Gen Virol68, 1695–1703, 1987.
CattaneoR., SchmidA., EschleD., BaczkoK., terMeulenV., and BilleterM.A., Cell55, 255–265, 1988.
BaczkoK., LiebertU.G., BilleterM., CattaneoR., BudkaH. and terMeulenV., J Virol59, 472–478, 1986.
LiebertU.G., BaczkoK., BudkaH., and terMeulenV., J Gen Virol67, 2435–2444, 1986.
HommaM., TashiroM., KonnoH., OharaY., HinoM. and TakaseS., Microbiol Immunol26, 1195–1202, 1982.
KomaseK., KasaokaT., YoshikawaY., SatoT.A. and YamanouchiK., Virus Genes4(2): 137–149, 1990.
EndersJ.F. and PeeblesT.C., Proc Soc Exp Biol Med86, 277–286, 1954.
TsukiyamaK., YoshikawaY. and YamanouchiK., Virology164, 523–530, 1988.
HeinkoffS., Gene28, 351–359, 1984.
SangerF., NicklenS. and CoulsonA.R., Proc Natl Acad Sci USA74, 5463–5467, 1977.
KozakM., Microbiol Rev47, 1–45, 1983.
KomaseK., HagaT., YoshikawaY., SatoT.A. and YamanouchiK., Virus Genes4(2): 163–172, 1990.
KozakM., Adv Virus Res31, 229–292, 1986.
PelletierJ. and SonenbergN., Cell40, 515–526, 1985.
SapJ., MnnozA., DammK., GoldbergY., GhysdaelJ., LeutzA., Nature (London)324, 635–640, 1986.
BarrettT., ClarkeD.K., EvansS.A. and RimaB.K., Virus Res8, 373–386, 1987.
HsuD., YamanakaM., MillerJ., DaleB., GrubmanM. and YilmaT., Virology166, 149–153, 1988.
WongT.C., AyataM., HiranoA., YoshikawaY., TsuruokaH. and YamanouchiK., J Virol63, 5464–5468, 1989.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Komase, K., Haga, T., Yoshikawa, Y. et al. Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis virus. IV. Nucleotide sequence of the fusion gene. Virus Genes 4, 173–181 (1990). https://doi.org/10.1007/BF00678408
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00678408