Abstract
P element mediated germ-line transformation was used to study the developmental specificity ofDrosophila chorion gene regulatory sequences directing expression of the bacterial reporter genes for chloramphenicol acetyltransferase (CAT) and β-galactosidase (lacZ). DNA fragments containing 5′ flanking plus the entire 5′ untranslated and the beginning of the coding region of either thes36 or thes15 chorion gene are able to confer on the reporter genes normal tissue as well as temporal specificity of expression, exclusively in the ovary of transformed female flies. However, if 5′ untranslated and coding regions are omitted, normal ovarian expression is maintained but tissue specificity is relaxed: expression of the reporter gene is detected both in the ovary and in specific non-ovarian tissues of transformed females and males. The evidence suggests that the missing 5′ untranslated and coding sequences may include negative elements that normally suppress expression in non-ovarian tissues, and that these putative elements are distinct from those that prevent premature expression in the ovarian follicles. The exact location of ectopiclacZ expression within the internal male genitalia depends on the constellation of 5′ flanking chorion regulatory sequences included in theP element constructs. Ectopic expression of theCAT gene in the male genitalia unders15 promoter control can be abolished by mutating the hexamer TCACGT, a sequence previously shown to be essential for the normal expression of this chorion gene in the ovary.
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by A. Spradling
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Bienz-Tadmor, B., Tolias, P., Stebbins-Boaz, B. et al. Chorion genecis-regulatory DNA restricts tissue specificity of reporter gene expression in transformedDrosophila . Chromosoma 101, 538–548 (1992). https://doi.org/10.1007/BF00660313
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DOI: https://doi.org/10.1007/BF00660313