Summary
The distribution of FMRFamide-like immunoreactivity was investigated in the brain of a myxinoid, the Pacific hagfish,Eptatretus stouti, by means of immunocytochemistry. In the forebrain, labelled cell bodies occurred in the infundibular nucleus of the hypothalamus and some closely adjacent nuclei. Labelled fibers formed a diffuse network in the forebrain, but there was no evidence for the presence of intracerebral ganglionic cells of the terminal nerve or a central projection of the terminal nerve. In the hindbrain, a group of labelled cells was found in the trigeminal sensory nucleus. A distinet terminal arborization occurred in the ventrally adjacent nucleus A of Kusunoki and around the nuclei of the branchial motor column. These findings suggest that FMRFamide may play a role in the central control of branchiomotor activity.
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References
Adams JC (1981) Heavy metal intensification of DAB-based HRP reaction product. J Histochem Cytochem 29:775
Bonn U, König B (1988) FMRFamide-like immunoreactivity in the brain and pituitary ofXenotoca eisenii: (Cyprinidontoformes, Teleostei). J Hirnforsch 29:121–131
Bonn U, König B (1989a) FMRFamide-like immunoreactivity in the brain and pituitary of the teleost,Eigenmannia lineata (Gymnotiformes). Z Mikr Anat Forsch 103:221–236
Bonn U, König B (1989b) FMRFamide-like immunoreactivity in the brain and pituitary ofCarassius auratus (Cyprinidae, Teleostei). J Hirnforsch 30:361–370
Chiba A, Oka S, Honma Y (1991) Immunocytochemical distribution of FMRFamide-like substance in the brain of the Cloudy dogfish,Scyliorhinus rorazame. Cell Tissue Res 265:243–250
Hofmann MH, Meyer DL (1991) Subdivisions of the terminal nerve inXenopus laevis. J Exp Zool 259:324–329
Hofmann MH, Meyer DL (1992) Peripheral origin of olfactory nerve fibers by-passing the olfactory bulb inXenopus laevis. Brain Res (in press)
Jansen J (1930) The brain ofMyxine glutinosa. J Comp Neurol 49:359–507
Jirikowski G, Erhart G, Grimmelikhuijzen CJP, Triepel J, Patzner RA (1984) FMRF-amide-like immunoreactivity in the brain and pituitary of the hagfishEptatretus burgeri (Cyclostomata). Cell Tissue Res 237:363–366
Kishida R, Onishi H, Nishizawa H, Katoda T, Goris RC, Kusunoki T (1986) Organization of the trigeminal and facial motor nuclei in the hagfish,Eptatretus burgeri: A retrograde HRP study. Brain Res 385:263–272
Kusunoki T, Katoda T, Kishida R (1982) Chemoarchitectonics of the brain stem of the hagfish,Eptatretus burgeri, with special reference to the primordial cerebellum. J Hirnforsch 23:109–119
Münz H, Stumpf WE, Jennes L (1981) LHRH systems in the brain of platyfish. Brain Res 221:1–13
Münz H, Claas B, Stumpf WE, Jennes L (1982) Centrifugal innervation of the retina by luteinizing hormone releasing hormone (LHRH)-immunoreactive telencephalic neurons in teleostean fishes. Cell Tissue Res 222:313–323
Muske LE, Moore FL (1988) The nervus terminalis in amphibians: anatomy, chemistry, and relationship with the hypothalamic gonadotropin-releasing hormone system. Brain Behav Evol 32:141–150
Nishizawa H, Kishida R, Katoda T, Goris RC (1988) Somatotopic organization of the primary sensory trigeminal neurons in the hagfish,Eptatretus burgeri. J Comp Neurol 267:281–295
Northcutt RG (1985) The brain and the sense organs of the earliest cranites: reconstruction of a morphotype. In: Foreman RE, Gorbman A, Dodd JM, Olsson R (eds) Evolutionary Biology of Primitive Fishes. Plenum Press, New York London, pp 81–112
Northcutt RG, Butler AB (1991) Retinofugal and retinopetal projections in the Green sunfish,Lepomis cyanellus. Brain Behav Evol 37:333–354
Östholm T, Ekström P, Ebbesson SOE (1990) Distribution of FMRFamide-like immunoreactivity in the brain, retina and nervus terminalis of the sockeye salmon parr,Oncorhynchus nerea. Cell Tissue Res 261:403–418
Ohtomi M, Fuji K, Kobayashi H (1989) Distribution of FMRFamide-like immunoreactivity in the brain and neurohypophysis of the lamprey,Lampetra japonica. Cell Tissue Res 256:581–584
Price DA, Greenberg MJ (1977) Structure of a molluscan cardioex-citatory neuropeptide. Science 197:670–671
Ronan M (1988) The sensory trigeminal tract of Pacific higfish. Brain Behav Evol 32:169–180
Rusoff AC, Hapner SJ (1990) Organization of retinopetal axons in the optic nerve of the cichlid fish,Herotilapia multispinosa. J Comp Neurol 294:418–430
Stell WK, Walker SE, Chohan KS, Ball AK (1984) The goldfish nervus terminalis: a luteinizing hormone-releasing hormone and molluscan cardioexcitatory peptide immunoreactive olfactoretinal pathway. Proc Natl Acad Sci USA 81:940–944
Sternberger LA (1979) Immunocytochemistry. Wiley, New York
Szabo T, Blähser S, Denizot J-P, Ravaille-Veron M (1991) The olfactoretinalis system=terminal nerve? NeuroReport 2:73–76
Triepel J, Grimmelikhuijzen CJP (1984) A critical examination of the occurrence of FMRFamide immunoreactivity in the brain of guinea pig and rat. Histochemistry 80:63–71
Uchiyama H (1990) Immunohistochemical subpopulations of retinopetal neurons in the nucleus olfactoretinalis in a teleost, the Whitespotted greenling (Hexagrammos stelleri). J Comp Neurol 293:54–62
Uchiyama H, Reh TA, Stell WK (1988) Immunocytochemical and morphological evidence for a retinopetal projection in anuran amphibians. J Comp Neurol 274:48–59
Wicht H, Northcutt RG (1990) Retinofugal and retinopetal projections in the Pacific hagfish,Eptatretus stouti. Brain Behav Evol 36:315–328
Wicht H, Northcutt RG (1992) The forebrain of the Pacific hagfish: A cladistic reconstruction of the ancestral craniate forebrain. Brain Behav Evol 40:25–64
Williams RG, Dockray GJ (1983) Immunohistochemical studies of FMRF-amide-like immunoreactivity in rat brain. Brain Res 267:213–229
Wirsig-Wiechmann CR (1990) The nervus terminalis in the chick: a FMRFamide-immunoreactive and AChE-positive nerve. Brain Res 523:175–179
Wirsig-Wiechmann CR, Basinger SF (1988) FMRFamide-immunoreactive fibers in the frog,Rana pipiens: demonstration by lesion and immunocytochemical techniques. Brain Res 449:116–133
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Wicht, H., Northcutt, R.G. FMRFamide-like immunoreactivity in the brain of the Pacific hagfish,Eptatretus stouti (Myxinoidea). Cell Tissue Res. 270, 443–449 (1992). https://doi.org/10.1007/BF00645045
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DOI: https://doi.org/10.1007/BF00645045