Abstract
Oligodeoxyribonucleotides containing 4-N-(ω-aminohexyl)-5-methyldeoxycytidine have been synthesized. It has been shown that a method of preparation based on the use of a monomeric component containing 4-N-(ω-aminohexyl)-5-methyldeoxycytidine is more reliable and promising than transamination at the level of an oligonucleotide containing 5-methyl-4-triazolyl-6-deoxythymidine. A structure of the monomeric component of oligonucleotide synthesis bearing a hexylamine grouping with trifluoroacetyl protection in the aliphatic amino group has been put forward. The structure of the modified deoxyribonucleotides obtained was confirmed not only by the method of chemical modification, degradation, and exhaustive enzymatic hydrolysis but also by obtaining derivatives at the aliphatic amino group with fluorescein isothiocyanate.
Similar content being viewed by others
Abbreviations
- Tri:
-
1,2,4-triazole
- MMTr:
-
monomethoxytrityl
- iPr:
-
isopropyl
- Py:
-
pyridine
- DMS:
-
dimethyl sulfate
- DEPC:
-
diethyl pyrocarbonate
Literature cited
J. M. Coull, H. L. Weith, and R. Bischoff, Tetrahedron Lett.,27, 3991 (1986).
P. Li, P. P. Medon, D. C. Skingle, J. A. Lanser, and R. H. Symons, Nucleic Acids Res.,15, 5275 (1987).
B. Arcangioli, S. Pochet, R. Sousa, and T. Haynh-Dinh, Eur. J. Biochem.,179, 359 (1989).
S. M. Gryaznov, I. P. Chernov, V. K. Potapov, A. A. Purmal', V. G. Metelev, A. A. Elov, and Z. A. Shabarova, Bioorg. Khim.,12, 1604 (1986).
A. M. Maxam and W. Gilbert, Methods Enzymol.,65, 499 (1980).
A. Rosenthal, F. Schubert, D. Cech, T. S. Orezkaja [Oretskaya], S. A. Kuznezova, and Z. A. Shabarova, Biomed. Biochim., Acta,44, 75 (1985).
S. Agrawal, C. Christodoulou, and M. J. Gait, Nucleic Acids Res.,14, 6227 (1986).
T. R. Webb and M. D. Matteucci, Nucl. Acids Res.,14, 7661 (1986).
W. L. Sung, J. Org. Chem.,47, 3623 (1982).
T. Atkinson and M. Smith, in: Oligonucleotide Synthesis: A Practical Approach, J. J. Gait (ed)., IRL Press, Oxford (1984), pp. 35–81.
E. M. Volkov, E. A. Romanova, A. Krug, T. S. Oretskaya, V. K. Potapov, and Z. A. Shabarova, Bioorg. Khim.,14, 1034 (1988).
Additional information
M. V. Lomonosov Moscow State University. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 698–704, September–October, 1991.
Rights and permissions
About this article
Cite this article
Volkov, E.M., Kubareva, E.A., Tashlitskii, V.N. et al. Synthesis of oligodeoxyribonucleotides containing 4-N-(ω-aminohexyl)-5-methyldeoxycytidine. Chem Nat Compd 27, 615–620 (1991). https://doi.org/10.1007/BF00630367
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00630367