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Comparative investigation of the structure of complexes of DNA with the polypeptide fragment 152–184 of histone H1 and the polypeptide (Lys-Ala-Ala)n

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Abstract

The interactions with DNA of a synthetic peptide fragment of histone H1 33 amino acid residues long the sequence of which corresponds to fragment 152–184 and of a synthetic polypeptide (Lys-Ala-Ala)n modeling the properties of the C-terminal fragments of histone H1 have been studied by the CD method. The use of the CD method for recording the spectra of the polypeptides under investigation in the free state and of the artificial complexes of DNA formed with them has permitted the characterization of different structural possibilities of the polypeptides in solution and of their complexes with DNA, and the conformational changes of the components of the complex under various ionic conditions.

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V. I. Nikitin Institute of Chemistry, Academy of Sciences of the Tadzhik SSR, Dushanbe, Institute of Cytology, Academy of Sciences of the USSR, Leningrad. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 798–805, November–December, 1990.

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Kamilova, R.R., Ramm, E.I., Ivanov, G.S. et al. Comparative investigation of the structure of complexes of DNA with the polypeptide fragment 152–184 of histone H1 and the polypeptide (Lys-Ala-Ala)n . Chem Nat Compd 26, 680–686 (1990). https://doi.org/10.1007/BF00630081

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  • DOI: https://doi.org/10.1007/BF00630081

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