Abstract
The conditions have been investigated for the potentiometric titration of N-acyl-2′-deoxyribonucleotides and of trityl carbinol derivatives and their two-component mixtures with a nitromethane solution of perchloric acid. The influence of water, acetone, chloroform, and acetic acid on the conditions for the acidimetric analysis of tri-p-methyltrityl carbinol, p-monomethoxytrityl carbinol, and di-p-methoxy trityl carbinol in nitromethane have been shown. Procedures have been developed for the quantitative determination of N6-benzoyl-5′-di-p-methoxytrityl-2′-deoxyriboadenosine, the 5′-tri-p-methyltrityl and 5′-p-monomethoxytrityl derivatives of N4-benzoyl-2′-deoxyribocytidine, of N6-benzoyl-2′-deoxyriboadenosine, and of N2-isobutyryl-2′-deoxyriboguanosine by differentiated potentiometric titration. For the determination of the 5′-di-p-methoxy derivatives of N4-benzoyl-2′-deoxyribocytidine and of N6-benozyl-2′-deoxyriboadenosine a procedure is proposed which includes the use of two parallel titrations. Methods have been developed for the use of milligram amounts of substance.
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All-Union Scientific-Research Institute of Applied Biochemistry and Biolar Scientific Production Amalgamation. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 718–723, September–October, 1987.
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Veveris, A.Y., Spintse, B.A., Luse, I.A. et al. Potentiometric differentiated titration of the components of nucleic acids and their derivatives. VII. Acidimetric determination of some N-acyl-2′-deoxyribonucleosides and their 5′-tritylated derivatives. Chem Nat Compd 23, 599–603 (1987). https://doi.org/10.1007/BF00598683
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DOI: https://doi.org/10.1007/BF00598683