Abstract
We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.
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Saarnak, A.E., Rodrigues, T., Schwartz, J. et al. Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue. Laser Med Sci 13, 22–31 (1998). https://doi.org/10.1007/BF00592957
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DOI: https://doi.org/10.1007/BF00592957