Skip to main content
SpringerLink
Log in

A technique for recording local blood flow and neuronal activity with a single microelectrode

  • Letters and Notes
  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Summary

A technique is presented for recording both local blood flow, by means of hydrogen clearance method, and neuronal activity of the cat's cortex using a single microelectrode. The requirements of electrodes for recording cerebral blood flow are different from those for recording extracellular spikes. Only electrodes within a small range of impedance (measured at 10 Hz) are suitable to record both. A bridge method is used for the recordings. The bridge signal is split in order to amplify cell activities and H2 responses separately. Three examples demonstrate the method and the importance of appropriately selected electrodes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aukland, K., Bower, B. F., Berlinger, R. W.: Measurement of local blood flow with hydrogen gas. Circulation Res.14, 164–187 (1964)

    Google Scholar 

  • Gesteland, R. C., Howland, B., Lettvin, J. Y., Pitts, W. H.: Comments on microelectrodes. Proc. IRE 1856–1862 (1959)

  • Green, J. D.: A simple microelectrode for recording from the central nervous system. Nature182, 962 (1958)

    Google Scholar 

  • Guld, Ch.: A glass covered platinum microelectrode. Med. Electron. Biol. Engng.2, 317–327 (1964)

    Google Scholar 

  • Heiss, W.-D., Hayakawa, T., Waltz, A. G.: Cortical neuronal function during ischemia. Effects of occlusion of one middle cerebral artery on single unit activity in cats. Arch. Neurol. (Chicago) in press (1976)

  • Hyman, E. S.: Linear System for quantitating hydrogen at a platinum electrode. Circulation Res.9, 1093–1097 (1961)

    Google Scholar 

  • Pasztor, E., Symon, L., Dorsch, N. W. C., Branston, N. M.: The hydrogen clearance method in assessment of blood flow in cortex, white matter and deep nuclei of baboons. Stroke4, 556–567 (1973)

    Google Scholar 

  • Rappelsberger, P.: Registrierung von Aktionspotentialen im Gehirn. Diplomarbeit, Technische Universität Wien (1968)

  • Robinson, D. A.: The electrode properties of metal microelectrodes. Proc. IEEE56, 1065–1071 (1968)

    Google Scholar 

  • Wolbarsht, M. L., MacNichol, E. F., Wagner, H. G.: Glass insulated platinum micro-electrode. Science132, 1309–1310 (1960)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Hirnkreislauflaboratorium der Neurologischen Universitätsklinik, Institut für Allgemeine und Vergleichende Physiologie der Universität Wien, A-1090, Wien, Austria

    P. Rappelsberger, W. -D. Heiss, R. Vollmer & M. Turnheim

  2. Hirnforschungsinstitut der Österreichischen Akademie der Wissenschaften, Neurophysiologisches Institut der Universität Wien, Währingerstrasse 17, A-1090, Wien, Austria

    P. Rappelsberger, W. -D. Heiss, R. Vollmer & M. Turnheim

Authors
  1. P. Rappelsberger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. -D. Heiss
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Vollmer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Turnheim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rappelsberger, P., Heiss, W.D., Vollmer, R. et al. A technique for recording local blood flow and neuronal activity with a single microelectrode. Pflügers Arch. 369, 183–186 (1977). https://doi.org/10.1007/BF00591575

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00591575

Key words

Search

Navigation