Summary
The formation of primary (rouleaux) and secondary (rouleaux networks) RCA was studied by microcinematography (12 frames/sec) and photometry in a counterrotating “rheoscope” chamber. The blood was first subjected to rapid viscometric flow (460 sec−1, all RBC dispersed and aligned in flow) and then brought abruptly to full stop. In normal human blood, primary and secondary RCA occurred simultaneously, and were completed within 8 to 10 sec after stop. Blood from pregnant women at term, known for its pronounced red cell aggregation, shows a dissociation between the formation of short primary rouleaux (initiated even before full stop and completed 1–2 sec thereafter) and secondary RCA completed 3–5 sec after stop. RCA increases the light transmission of blood (measured by an increase in photovoltageV), the process and its first derivative (dV/dt=İ) can be recorded. After flow stop, there is an exponential decay of İ(İ=tİ0·e−λt). The half time of this decay is recorded and correlated to the kinetics of red cell aggregate formation In human blood the half time of this process varies between 1.0 and 6.0 sec. In suspensions of human RBC in artificial plasmas,t 1/2 decreases with increasing concentration of fibrinogen and/or Dextran 25000, the second component appearing at concentrations above 500 mg-%. The method lend sitself for the quantification of RCA in small blood samples (20 μl).
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References
Berman, H. J., Fuhro, R. I.: Quantitative red blood cell aggregometry of human and hamster bloods. Proc. VIIth Conference on Microcirculation. (Aberdeen 1972), pp. 117–125, Basel: Karger 1973
Brinkman, R., Zijlstra, W. G., Jansonius, N. J.: Quantitative evaluation of the rate of rouleaux formation of erythrocytes by measuring light reflection (“Syllectometry”). Proc. kon. ned. Akad. Wet., Ser. C66, 236–248 (1963)
Chien, S.: The present state of blood rheology. In: Hemodilution, theoretical basis and clinical application. K. Messmer and H. Schmid-Schönbein, eds., pp. 1–40, Basel: Karger 1972
Chien, S., Usami, S., Taylor, H. M., Lundberg, J. L., Gregersen, M. I.: Effect of hematocrit and plasmaproteins on human blood rheology at low rates of shear. J. appl. Physiol.21, 81–87 (1966)
Dintenfass, L.: Blood microrheology—viscosity factors in blood flow, ischemia and thrombosis. London: Butterworths 1971
Dognon, A.: Granulométric optique de la suspension sanguine. Nouv. technique d'étude C.R.268, 974–975 (1969)
Fahraeus, R.: Suspension-stability of the blood. Acta med. scand.55, 1–228 (1921)
Healy, J. C.: Etude expérimentale des associations réversibles entre les globules rouges. Thèse de doctorat, Univ. Paris VI, 1973
Klose, H. J., Volger, E., Brechtelsbauer, H., Heinrich, L., Schmid-Schönbein, H.: Microrheology and light transmission of blood. I. The photometric effects of Microrheology and light transmission of blood. I. The photometric effects of red cell aggregation and red cell orientation. Pflügers Arch.333, 126–139 (1972)
Merrill, E. W.: Rheology of blood. Physiol. Rev.49, 863–888 (1969)
Schmid-Schönbein, H., Gallasch, G., Volger, E., Klose, H. J.: Microrheology and protein chemistry of pathological red cell aggregation (blood sludge) studied in vitro. Biorheology10, 213–227 (1973)
Schmid-Schönbein, H., Gosen, J. v., Heinich, L., Klose, H. J., Volger, E.: A counter-rotating “rheoscope chamber” for the study of the microrheology of blood cell aggregation by microscopic observation and microphotometry. Microvasc. Res.6, 366–376 (1973)
Schmid-Schönbein, H., Volger, E., Klose, H. J.: Microrheology and light transmission of blood. II. The photometric quantification of red cell aggregate formation and dispersion in flow. Pflügers Arch.333, 140–155 (1972)
Schmid-Schönbein, H., Wells, R. E.: Rheological properties of human erythrocytes and their influence upon the anomalous viscosity of blood. Ergebn. Physiol.63, 146–219 (1971)
Usami, S., Chien, S.: Optical reflectometry of red blood cell aggregation under shear flow. Proc. VIIth Conference on Microcirculation (Aberdeen 1972), pp. 91–98. Basel: Karger 1973
Volger, E., Schmid-Schönbein, H., Gosen, J. v., Klose, H. J. Kline, K. A.: Microrheology and light transmission of blood. IV. The kinetics of artificial red cell aggregation induced by dextran. Pflügers Arch.354, 319–337 (1975)
Wells, R. E., Jr., Gawronski, T. H., Cox, P. M., Perera, R. D.: Influence of fibrinogen on flow properties of erythrocyte suspensions. Amer. J. Physiol.207, 1035–1040 (1964)
Zijlstra, W. G., Heeres, S. G.: The influence of plasma substitutes on the suspension stability of human blood. Proc. kon. ned. Akad. Wet., Ser. C68, 413–422 (1965)
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Supported by Deutsche Forschungsgemeinschaft.
Supported by a Senior Fellowship award of the Alexander v. Humboldt-Stiftung.
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Schmid-Schönbein, H., Kline, K.A., Heinich, L. et al. Microrheology and light transmission of blood. Pflugers Arch. 354, 299–317 (1975). https://doi.org/10.1007/BF00587849
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DOI: https://doi.org/10.1007/BF00587849