Abstract
Voltage-dependent inward Ba++ and Ca++ currents were recorded in cultured neonatal rat pancreatic islet cells using the whole-cell voltage clamp technique. Outward current was suppressed by internal Cs+ and ATP and external TEA. Inward currents activated rapidly and decayed to a variable extent. The current decay was particularly marked when using long duration or large depolarizing pulses. Currents were due to Ca++ channel activation since they were abolished by omitting Ba++ and Ca++ or including Co++.
This is a preview of subscription content, log in via an institution to check access.
References
Cook, D.L., Crill, W.E. and Porte D., Jr. Plateau potentials in pancreatic islet cells are voltage-dependent action potentials. Nature286: 404–406 (1980).
Cook, D.L., Electrical pacemaker mechanisms of pancreatic islet cells. Fed. Proc.43:2368–2372 (1984).
Cook, D.L., Ikeuchi, M. and Fujimoto, F. Lowering of pH inhibits Ca++-activated K+ channels in pancreatic B-cells. Nature311:269–271 (1984).
Cook, D.L., Hales, C.N. Intracellular ATP directly blocks K+ channels in pancreatic B-cells. Nature311:271–273 (1984).
Dean, P.M. and Matthews, E.K. Electrical activity in pancreatic islet cells: effect of ions. J. Physiol.210:265–275 (1970).
Fenwick, E.M., Marty, A. and Neher, E. Sodium and calcium channels in bovine chromaffin cells. J. Physiol.331:599–635 (1982).
Fujimoto, W. Purification of neonatal rat pancreatic monolayer cultures for endocrine cells. Proc. Soc. Exp. Biol. Med.162:241–244 (1979).
Fujimoto, W. and Metz, S. Phasic glucose-stimulated insulin secretion by neonatal rat pancreatic islet cells. Diabetes33:872–878 (1984).
Hagiwara, S., Byerly, L. Calcium channel. Ann. Rev. Neurosci.4:69–125 (1981).
Hamill, O.P., Marty, A., Neher, E., Sakmann, B. Sigworth, F.J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch.391:85–100 (1981).
Ikeuchi, M. and Yagi, K. Pancreatic A cell generates action potential. Jap. J. Physiol.32:873–878 (1982).
Marty, A. and Neher, E. Tight-seal whole cell recording, in:Single Channel Recording, B. Sakmann and E. Neher, eds. Plenum, New York, NY (1983).
Meissner, H.P. and Schmeer, W. The significance of calcium ions for the glucose-induced electrical activity of pancreatic B cells, in:The Mechanism of Gated Calcium Transport Across Biological Membranes, S. Ohnishi and M. Endo, eds. Academic Press, New York, NY (1981).
Ribalet, B. and Beigelman, P.M. Calcium action potentials and potassium permeability activation in pancreatic B-cells. Am. J. Physiol.239:C124–133 (1980).
Wollheim, C.B. and Sharp, W.G. Regulation of insulin release by calcium. Physiol. Revs.61:914–973 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Satin, L.S., Cook, D.L. Voltage-gated Ca2+ current in pancreatic B-cells. Pflugers Arch. 404, 385–387 (1985). https://doi.org/10.1007/BF00585354
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00585354