Abstract
The reabsorptive duct of the eccrine sweat gland has a large transepithelial conductance consisting mainly of a high conductance to Cl− and a smaller, amiloride-blockable Na+ conductance (Bijman and Frömter 1986; Quinton 1985). Cells have been cultured from sweat ducts and their properties previously studied in Ussing chambers (Pedersen 1988) and with microelectrodes (Jones et al. 1988). We have now studied the ion channels present in excised, inside-out patches of human cultured sweat duct cells, and find a marked predominance of linear, 16 pS, amiloride-blockable, low selectivity, Na+ channels. Such channels were seen in 54/92 (59%) of the patches, with up to 7 channels recorded in a single patch.Other channel types were seen at much lower densities. The prevalence of an amiloride-blockable Na+ channel in cultured duct cells clearly distinguishes these cells from cultured sweat gland secretory cells, which lack such a channel.
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References
Bijman, J., Frömter, E. (1986) Direct demonstration of high transepithelial chloride conductance in normal human sweat duct which is absent in cystic fibrosis. Pflüg. Arch. 407:S123-S127
Bijman, J., Quinton, P. M. (1987) Permeability properties of cell membranes and tight junctions of normal and cystic fibrosis sweat ducts. Pflüg. Arch. 408:505–510.
Bijman, J., Kansen, M., Hoogeveen, A. H., Scholte, B., van der Kamp, A., de Jonge, H. (1988) Electrolyte transport in normal and CF epithelia. In: Wong, P.Y.D., Young, J.A. (eds) Exocrine Secretion. Hong Kong University Press, Hong Kong, pp. 17–19.
Garty, H., Benos, D.J. (1988) Characteristics and regulatory mechanisms of the amiloride-blockable Na+ channel. Physiol. Rev. 68:309–373.
Hamilton, K.L., Eaton, D.C. (1985) Single channel recordings from two types of amiloride-sensitive epithelial Na+channels. Membr. Biochem. 6: 149–171.
Hanrahan, J.W. (1987) Anion conductance in cultured sweat gland cells. Ped. Pulmonol. 1:49.
Jones, C.J., Bell, C.L., Quinton, P.M. (1988) Different physiological signatures of sweat gland secretory and duct cells in culture. Am. J. Physiol. 255: (Cell Physiol. 24):C102-C111.
Pedersen, P.S. (1988) The study of epithelial function by in vitro culture of sweat duct cells. In: Mastella, G., Quinton, P.M. (eds) Cellular and Molecular Basis of Cystic Fibrosis. San Francisco Press, San Francisco, pp. 395–401.
Quinton, P.M. (1986) Missing Cl conductance in cystic fibrosis. Am. J. Physiol. 251:C649-C652
Reddy, M.M., Riordan, J.R., Quinton, P.M. (1988) Electrical properties of cultured reabsorptive sweat duct cells from normal and cystic fibrosis subjects: intracellular microelectrode analysis. In: Mastella, G., Quinton, P.M. (eds) Cellular and Molecular Basis of Cystic Fibrosis. San Francisco Press, San Francisco, pp. 383–393.
Welsh, M.J., McCann, J.D., Dearborn, D.G. (1987) Chloride and sodium channel currents in normal and cystic fibrosis sweat duct epithelium. Fed. Proc. 46:1271
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Joris, L., Krouse, M.E., Hagiwara, G. et al. Patch-clamp study of cultured human sweat duct cells: amiloride-blockable Na+ channel. Pflugers Arch. 414, 369–372 (1989). https://doi.org/10.1007/BF00584641
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DOI: https://doi.org/10.1007/BF00584641