Abstract
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1.
The isolated midgut of a lepidopteran larva (Manduca sexta), 5th instar was investigated with voltageclamp and fluctuation analysis techniques.
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2.
With high K+ insect saline on both sides the outward-directed short-circuit current (I sc) was carried by K+ (I K) from serosal to mucosal compartment.I K could be blocked, in a dose-dependent manner by serosal Ba2+ ions. There was no current with serosal Na+.
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3.
Noise analysis ofI K revealed a Lorentzian component in the power spectrum when Ba2+ was present in the serosal solution. The Ba2+/receptor kinetics show pseudo-first order characteristics only at low [Ba2+]s. For [Ba2+]s>K Ba, the apparrent Ba2+ association rate decreases with a hyperbolic course as a function of serosal [Ba2+] which could indicate some “substrate-inhibition”-like interaction of Ba2+ at its receptor site.
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4.
It is concluded that the serosal membranes of the K+-secreting intestinal cells contain the common type of Ba2+-blockable K+ channel which provides the serosal pathway for K+ during secretion which is ultimately driven by the mucosally-located electrogenic K+-ATPase.
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Zeiske, W., Van Driessche, W. & Ziegler, R. Current-noise analysis of the basolateral route for K+ ions across a K+-secreting insect midgut epithelium (Manduca sexta). Pflugers Arch. 407, 657–663 (1986). https://doi.org/10.1007/BF00582648
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DOI: https://doi.org/10.1007/BF00582648