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The effect of streptozotocin-induced diabetes mellitus on urinary excretion of sodium and renal Na+−K+-ATPase activity

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

Renal sodium handling and microsomal Na+−K+-ATPase activity in kidney cortex, medulla and papilla of rats with streptozotocin-induced diabetes mellitus (DM) was studied.

During 7 days following the administration of streptozotocin GFR, urinary excretion, filtered load and tubular reabsorption of Na+ averaged (mean±SE) 1.18±0.016 ml/min, 1.74±0.14, 177.3±8.9 and 175.6±8.9 mEq/min respectively in experimental rats as compared to corresponding rates of 0.85±0.04 (P<0.001), 0.85±0.03 (P<0.001), 129.8±5.8 (P<0.001) and 129±5.8 (P<0.001) respectively in the control rats.

The activity of microsomal Na−K-ATPase in the kidney cortex, medulla and papilla of the control group was (mean±SE) 44.7±1.7, 150±7.5 and 37.4±3.6 (μmoles Pi/mg prot/h) respectively. 24 h after DM induction Na−K-ATPase activity in the cortex rose to 59.3±2.4 (P<0.001) and remained high after 3 and 7 days. Medullary Na−K-ATPase activity was unchanged 24 h after streptozotocin administration but was markedly increased to 260±9 (P<0.001) after 3 days and remained high after 7 days.

These findings show that stretozotocin-induced DM in rats causes a substantial increase in GFR which is associated with a net increase in filtered and reabsorbed load of Na+ and natriuresis. These alterations are accompanied by a marked increase in Na−K-ATPase activity in renal medulla and in the cortex.

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Authors and Affiliations

  1. Nephrology Services, Hadassah Hebrew University Hospital, Kiryat Hadassah, PO. Box 12000, IL-91120, Jerusalem, Israel

    Hanna Wald & Mordecai M. Popovtzer

Authors
  1. Hanna Wald
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  2. Mordecai M. Popovtzer
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This study was supported by the Morton S. Kaufman Hemodialysis Foundation and by the Joint Research Fund of the Hebrew University and Hadassah

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Wald, H., Popovtzer, M.M. The effect of streptozotocin-induced diabetes mellitus on urinary excretion of sodium and renal Na+−K+-ATPase activity. Pflugers Arch. 401, 97–100 (1984). https://doi.org/10.1007/BF00581539

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  • DOI: https://doi.org/10.1007/BF00581539

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