Summary
Small columns packed with octadecasilylsilica were used to extract arginine-vasopressin in femtomole amounts from biological fluids for radioimmunoassay. This method is effective in isolating the peptide from substances in plasma and serum which interfere with its quantitation. Consistent and reproducible results and recoveries >80% were obtained with this procedure. High pressure liquid chromatography and serial dilution of extracted samples confirm the identity of the extracted product.
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Bennett, H.P.J., Hudson, A.M., McMartin, C., Purdon, G.E.: Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Biochem. J. 168, 9–13 (1977).
Czernichow, P., Patin, A.M., Pomarede, R.: Identification of a macro-molecular form of immuno-reactive arginine vasopressin in human plasma. Program of the 61st Annual Meeting of the Endocrine Society, p. 96 (1979).
Edwards, C.R.W., Chard, T., Kitau, M.J., Forsling, M.L.: The development of a radioimmunoassay and a plasma extraction method for vasopressin. J. Endocrinol. 4, xi-xii (1970).
Johnston, C.I.: Radioimmunoassay for plasma antidiuretic hormone. J. Endocrinol. 52, 69–78 (1972).
Kelley, L., Bennett, H.P.J., Hudson, A.M., McMartin, C., Purdon, G.E.: An efficient method for the extraction of undamaged peptides from various tissues. J. Endocrinol. 77, 24P (1978).
Moore, G., Lutterodt, A., Burford, G., Lederis, K.: A highly specific antiserum for arginine vasopressin. Endocrinology 101, 1421–1435 (1977).
Morton, J.J., Riegger, A.J.G.: A novel extraction method for plasma vasopressin and its application in a radioimmunoassay. J. Endocrinol. 77, 277–278 (1978).
North, W.G., LaRochelle, F.T., Jr., Haldar, J., Sawyer, W.H., Valtin, H.: Characterization of an antiserum used in a radioimmunoassay for arginine-vasopressin: Implications for reference standards. Endocrinology 103, 1976–1984 (1978).
O'Hare, M.J., Nice, E.C.: Hydrophobic high-performance liquid chromatography polypeptides and proteins on alkylsilane-bonded silica. J. Chromatography 171, 209–226 (1979).
Robertson, G.L., Klein, L.A., Roth, J., Gorden, P.: Immunoassay of plasma vasopressin in man. Proc. Nat. Acad. Sci. (USA) 66, 1298–1305 (1970).
Robertson, G.L., Mahr, E.A., Athar, S., Sinha, T.: Development and clinical application of a new method for the radioimmunoassay of arginine vasopressin in human plasma. J.C.I. 52, 2340–2352 (1973).
Robinson, A.G., Frantz, A.G.: Radioimmunoassay of Posterior Pituitary Peptides: A Review. Metabolism 22, 1047–1057 (1973).
Robinson, I.C.A.F., Walker, J.M.: Extraction of small amounts of oxytocin from biological fluids by means of agarose-bound neurophysin J. Endocrinol. 80, 191–202 (1979).
Rodbard, D. and Lewald, J.E.: Computer analysis of radioligand assays and radioimmunoassay data. Karolinski Symposia, Research Methods in Reproductive Endocrinology, Geneva March 1970. Acta Endocrinol. 64, Suppl. 147, 79–103 (1970).
Skowsky, W.R., Rosenbloom, A.A., Fisher, D.A.: Radioimmunoassay measurement of arginine vasopressin in serum: Development and application J. Clin. Endocrinol. 38, 278–287 (1974).
Thomas, T.H., Lee, M.R.: The specificity of antisera for the radioimmunoassay of argininevasopressin in human plasma and urine during water loading and dehydration. Clin. Sci. Mol. Med. 51, 525–536 (1976).
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LaRochelle, F.T., North, W.G. & Stern, P. A new extraction of arginine vasopressin from blood: The use of octadecasilyl-silica. Pflugers Arch. 387, 79–81 (1980). https://doi.org/10.1007/BF00580849
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DOI: https://doi.org/10.1007/BF00580849