Summary
Hydra oligactis exposed to 3 μg/ml actinomycin D for 24 hours regenerated only the first pair of tentacles (the mid-laterals). If left uncut, actinomycin D treated animals underwent a reduction of the normal number of tentacles to two or less.
Inductive activity was retained in the 2-tentacled hypostomes. However, the tentacles present exhibited reduced capacities to capture and manipulate prey.
Histological studies showed that the tentacles of actinomycin D treated hydra were morphologically identical to those of the controls. The interstitial cell (I-cell) population of the treated animals, however, became depleted. Replacing the hypostome of an actinomycin D treated hydra with a normal hypostome reversed the cellular effects of actinomycin D treatment.
The modifications in tentacle morphogenesis occurring after actinomycin D treatment are consistent with an impairment of hypostomal function in the animal. It is suggested that the morphological site of this malfunction may be in the nervous system.
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Research supported by American Cancer Society Institutional Grant No. 342-9157, USPHS Institutional Grant No. 342-9241 and by a grant from Research Corporation.
Part of this work was completed while L.H. was an undergraduate research student supported by the National Science Foundation.
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Lesh-Laurie, G.E., Hang, L. Tentacle morphogenesis in hydra. W. Roux' Archiv f. Entwicklungsmechanik 169, 314–334 (1972). https://doi.org/10.1007/BF00580252
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DOI: https://doi.org/10.1007/BF00580252