Abstract
DNA samples from cancer tissues diagnosed histologically as squamous cell carcinoma of the uterine cervix were examined for the presence of human papillomavirus (HPV) genome DNA by Southern blot hybridization. Of 63 specimens, 32 were found to hybridize with HPV DNA probes; 23 specimens (35%) with HPV type 16 (HPV16), one (2%) with HPV type 18 (HPV18), four (7%) with HPV type 52b (HPV52b), and four others (7%) weakly with HPV52b. Specimens negative for HPV DNA with Southern blot hybridization were subjected to polymerase chain reaction (PCR) to determine the presence of HPV DNA under more sensitive conditions. After PCR using one set of primers specific for HPV16 and HPV52b, 7 out of 31 specimens were found to have HPV16 DNA. None was positive for HPV52b DNA. Our results indicate that HPV52b, as well as HPV16 and HPV18, is associated with squamous cell carcinoma of uterine cervix, and more sensitive determination of HPV infection can be made by amplification of the viral genome by PCR.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
BrokerT.R. and BotchanM. in BotchanM., GrodzickerT., and SharpP.A. (eds.)Cancer Cells/4, DNA Tumor Viruses. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1986, pp. 17–36.
Zur HausenH. in PetoR. and Zur HausenH. (eds.)Banbury Report 21: Viral Etiology of Cervical Cancer. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1986, pp. 327–332.
SaikiR.K., ScharfS., FaloonaF., MullisK.B., HornG.T., ErlichH.A., and ArnheimN., Science230, 1350–1354, 1985.
ShibataD.R., ArnheimN., and MartinW.J., J Exp Med167, 225–230, 1988.
ShibataD., FuY.S., GuptaJ.W., ShahK.V., ArnheimN., and MartinW.J., Lab Invest59, 555–559, 1988.
YoungL.S., BevanI.S., JohnsonM.A., BromidgeP.I., MaitlandN.J., and WoodmanC.B.J., Br Med J298, 14–18, 1989.
KiyabuM.T., ShibataD., ArnheimN., MartinW.J., and FitzgibbonsP.L., Am J Surg Pathol13, 221–224, 1989.
YajimaH., NodaT., VilliersE.M., YajimaA., YamamotoK., NodaK., and ItoY., Cancer Res48, 7164–7172, 1988.
TaseT., OkagakiT., ClarkB.A., ManiasD.A., OstrowR.S., TwiggsL.B., and FarasA.J., Cancer Res48, 993–998, 1988.
TaseT., SatoS., WadaY., YagimaA., and OkagakiT., Tohoku J Exp Med156, 47–53, 1988.
YaegashiN., ShiraishiH., TakeshitaT., NakamuraM., YajimaA., and SugamuraK. J Virol63, 2422–2426, 1989.
NodaT., YajimaH., and ItoY., J Virol62, 313–324, 1988.
VilliersE.M., J Virol63, 4898–4903, 1989.
GissmannL. and SchneiderA. in PetoR. and Zur HausenH. (eds.)Banbury Report 21: Viral Etiology of Cervical Cancer. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1986, pp. 217–224.
YoshikawaH., MatsukuraT., YamamotoE., KawanaT., MizunoM., and YoshiikeT., Jpn J Cancer Res76, 667–671, 1985.
SaitoJ., YutsudoM., InoueM., UedaG., TanizawaO., and HakuraA., Jpn J Cancer Res78, 1081–1087, 1987.
ShimodaK., LorinczA.T., TempleG.F., and LancasterW.D., J Gen Virol69, 2925–2928, 1988.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yaegashi, N., Yajima, H., Shikano, K. et al. Detection of human papillomavirus (HPV) type 16 and 52b in cervical cancer tissues by Southern blot hybridization and polymerase chain reaction (PCR). Virus Genes 4, 313–323 (1990). https://doi.org/10.1007/BF00570026
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00570026