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Comparison of gentamicin C1 and (C1a, C2)-levels in patients

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Summary

A high performance liquid chromatographic (HPLC) assay method has been used to measure gentamicin serum concentrations in patients receiving gentamicin complex. The HPLC method resolves gentamicin C1 from the other two components, C1a and C2; gentamicin C1a and C2 cochromatograph. In the analysis of 46 serum samples collected from 16 patients it was found that the mean ratio (PHR) of the peak height of gentamicin C1 to the height of the peak due to components C1a and C2 was 0.53±0.05; this value agreed well with the PHR's usually found from the HPLC analysis of aqueous solutions of gentamicin complex or of gentamicin dosage forms. In an additional two patients, the HPLC analysis of a sample of the gentamicin dosage form administered, a urine sample, and serum samples, resulted in almost identical PHR's for the respective patients. Finally, similar results were obtained from an experiment in a rabbit. It was concluded that the disposition of all three components of gentamicin complex are the same or very similar.

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References

  1. Mosegaard, A., Welling, P.G., Madsen, P.O.: Gentamicin and gentamicin C1 in the treatment of complicated urinary tract infections: Comparative study of efficacy, tolerance, and pharmacokinetics. Antimicrob. Agents Chemother.7, 328–332 (1975)

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Authors and Affiliations

  1. University of Illinois at the Medical Center, 60612, Chicago, Illinois, USA

    Roger L. Nation, Geoffrey W. Peng, Win L. Chiou & James Malow

  2. Clinical Pharmacokinetics Laboratory and Department of Pharmacy, College of Pharmacy, USA

    Win L. Chiou

  3. Section of Infectious Diseases, Department of Medicine, ALSM, University of Illinois College of Medicine, USA

    James Malow

Authors
  1. Roger L. Nation
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  2. Geoffrey W. Peng
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  3. Win L. Chiou
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  4. James Malow
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Nation, R.L., Peng, G.W., Chiou, W.L. et al. Comparison of gentamicin C1 and (C1a, C2)-levels in patients. Eur J Clin Pharmacol 13, 459–462 (1978). https://doi.org/10.1007/BF00566326

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  • DOI: https://doi.org/10.1007/BF00566326

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