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Epidermal nicotinamide adenine dinucleotides in psoriasis and neurodermatitis (Lichen Simplex Hypertrophicus)

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Summary

Epidermal nicotinamide adenine dinucleotides were measured in subcorneal and basal epidermal layers in patients with psoriasis and neurodermatitis and in healthy controls. Lowry's microtechniques utilizing enzymatic cycling were used. The total NAD content in these groups was except for the involved psoriatic skin about 1.1 mmoles/kg dry weight. Between 40 to 50% of this content consisted of the reduced form. In the involved psoriatic skin the total NAD content was increased to 1.5 mmoles/kg, this increase being mainly due to a rise in NAD+. There was no difference in NAD content between epidermal layers in the various groups studied.

The total NADP content was near 0.15 mmoles/kg in healthy controls and in patients with neurodermatitis. The subcorneal layers contained 10% more of the dinucleotide than the basal layers, but in the two layers the reduced form amounted to about 80% of the total. In both non-involved and involved skin of the psoriatic patients the total NADP content was significantly increased above the control level, by up to 20% in the former and up to 65% in the latter. In the two layers studied the NADP+ content was increased by about 75% in both non-involved and involved areas. In contrast, the NADPH content rose only in the basal layers of the lesion, by 55%.

The increased levels of NADP+ and NADPH found in psoriasis might suggest an accelerated or differently conducted NADPH dependent biosynthesis in this disease.

Zusammenfassung

Epidermale Nicotinamid-Adenin-Dinucleotide wurden in den subcornealen und basalen Epidermisabschnitten von Patienten mit Psoriasis und Neurodermitis sowie bei gesunden Kontrollpersonen bestimmt. Die Lowry-Mikrotechnik mit enzymatischem Cycling fand Verwendung. Der totale NAD-Gehalt in der Haut dieser Personen außer der erkrankten Haut von Psoriatikern war ungefähr 1,1 mmol/kg Trockengewicht. Etwa 40–50% von diesem Gehalt wurde in reduzierter Form gefunden. Der totale Gehalt der erkrankten Haut von Patienten mit Psoriasis war zu 1,5 mmol/kg erhöht. Die Vermehrung beruhte hauptsächlich auf einer Zunahme von NAD+. Es bestand kein Unterschied in dem NAD-Gehalt in den Epidermisabschnitten der verschiedenen untersuchten Gruppen.

Der totale NADP-Gehalt war nahe 0,15 mmol/kg in den gesunden Kontrollpersonen und in den Patienten mit Neurodermitis. Die subcornealen Epidermisabschnitte enthalten 10% mehr von dem Dinucleotid als die basalen Abschnitte, aber die reduzierte Form betrug etwa 80% von dem totalen Gehalt in den beiden untersuchten Abschnitten. Der totale NADP-Gehalt in der nichterkrankten wie in der erkrankten Haut von Patienten mit Psoriasis waren signifikant über das Kontrollniveau erhöht. Die Vermehrung betrug bis zu 20% in der ersteren und bis zu 65% in der letzteren. Der NADP+-Gehalt in der nicht erkrankten wie in der erkrankten Haut der Psoriatiker war gleichmäßig etwa 75% in den beiden untersuchten Epidermisabschnitten erhöht. Auf der anderen Seite war der NADPH-Gehalt nur zu 55% in den basalen Epidermisabschnitten erhöht.

Der erhöhte Gehalt von NADP+ und NADPH der in der Psoriasis gefunden wurde, ist ein Befund, der eine accelerierte oder anders geleitete NADPH-abhängige Biosynthese bei dieser Krankheit andeutet.

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Kindly supported by the Swedish Medical Research Council (X 19-2593), by the Foundation of Edvard Welander and of the Karolinska Institute, and by the Swedish Psoriasis Association, Stockholm.

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Hammar, H. Epidermal nicotinamide adenine dinucleotides in psoriasis and neurodermatitis (Lichen Simplex Hypertrophicus). Arch. Derm. Res. 252, 217–227 (1975). https://doi.org/10.1007/BF00557922

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