Zusammenfassung
Durch Abklemmen der Mesenterial- und Collateralgefäße an der anaesthesierten Ratte konnte für kurze Zeit eine akute Ischämie von Darmschlingen erzeugt werden.
Nach in vitro-Messungen der Resorptionskapazität genügt eine 10 min andauernde Ischämie, um die funktionelle Kapazität der untersuchten Darmschlinge signifikant zu senken. Nach 30 min dauernder Ischämie ist die resorptive Kapazität des Gewebes fast ganz verloren. Eine histologische Untersuchung ergibt eine voll-ständige Zerstörung der Mucosa. Die mitotische Aktivität der Krypten bleibt unbeeinflußt. Das Ausmaß der morphologischen Schädigung zeigt eine gute Korrelation zum Verlust der physiologischen Funktion.
Nach 30 min andauernder Ischämie zeigt das morphologische Bild innerhalb von 2 Tagen eine fast vollständige Regeneration der Zelle. Im allgemeinen sind funktionelle und strukturelle Wiederherstellung miteinander gekoppelt. In einigen Fällen kann eine verzögerte funktionelle Erholung beobachtet werden.
Werden die Mesenterialgefäße bei Erhaltung des Collateralkreislaufes abgebunden, so wird die funktionelle Aktivität erniedrigt, ohne daß gleichzeitig morphologische Schäden zu beobachten sind.
Perfusion einer ischämischen Darmschlinge mit O2-gesätt. Krebs-Bicarbonat-Puffer vermittelt einen gewissen Schutz gegen die Zerstörung des Gewebes.
Unter Berücksichtigung der einschlägigen Literatur wird eine mögliche Erklärung der geschilderten Befunde versucht.
Summary
Acute ischaemia of loops of rat intestine has been induced by clamping the mesenteric and collateral vessels of an anaesthetized animal for various periods of time.
10 min ischaemia is sufficient to lower significantly the functional capacity of the experimental loop, as witnessed by an in vitro absorption test. After 30 min ischaemia, the absorptive capacity is almost entirely destroyed. Histological examination reveals complete destruction of the villous architecture of the mucosa, though mitotic activity in the crypts remains unaffected. The degree of morphological damage shows a close correlation with the loss in functional viability.
Morphological recovery is essentially complete after two days following 30 min ischaemia. Functional recuperation generally coincides with structural recovery, though cases of retarded functional recuperation have occurred.
On ligating the mesenteric vessels without lesions of the collateral circulation, functional activity is depressed without concomitant morphological damage.
Perfusion of an ischaemic loop of intestine with oxygenated Krebs bicarbonate buffer provides a certain degree of protection against the destruction of the tissue.
A short review of the relevant literature is presented, and a tentative explanation of these findings is put forward.
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Robinson, J.W.L., Antonioli, JA. & Mirkovitch, V. The intestinal response to ischaemia. Naunyn-Schmiedebergs Arch. Pharmak. u. Exp. Path. 255, 178–191 (1966). https://doi.org/10.1007/BF00543211
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DOI: https://doi.org/10.1007/BF00543211