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Synthesis of functional bovine opsin in insect cells under control of the baculovirus polyhedrin promoter

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Abstract

In vitro expression of cDNA encoding bovine opsin is accomplished using the baculovirus expression vector system. Full-length opsin was synthesized which was recognized by poly- and monoclonal antisera raised against bovine rhodopsin. Upon infection with a recombinant virus, 1×106 insect cells produced up to 3 μg opsin. Incubation of the in vitro synthesized opsin with 11-cis retinal produced a hydroxylamine-stable, photosensitive pigment.

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Abbreviations

dpi:

days post infection

pfu:

plaque forming units

AcNPV:

Autographa californica nuclear polyhedrosis virus

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Authors and Affiliations

  1. Department of Biochemistry, Center of Eye Research, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands

    J. J. M. Janssen, W. A. H. M. van Groningen-Luyben & W. J. de Grip

  2. Dept. of Biochemistry, Molecular Oncology Section, University of Nijmegen, St. Adelbertuspl. 1, 6500 HB, Nijmegen, The Netherlands

    W. J. M. van de Ven

  3. Dept. of Virology, Agricultural University, 6709 PD, Wageningen, The Netherlands

    J. Roosien & J. M. Vlak

Authors
  1. J. J. M. Janssen
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  2. W. J. M. van de Ven
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  3. W. A. H. M. van Groningen-Luyben
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  4. J. Roosien
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  5. J. M. Vlak
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  6. W. J. de Grip
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Janssen, J.J.M., van de Ven, W.J.M., van Groningen-Luyben, W.A.H.M. et al. Synthesis of functional bovine opsin in insect cells under control of the baculovirus polyhedrin promoter. Mol Biol Rep 13, 65–71 (1988). https://doi.org/10.1007/BF00539052

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  • DOI: https://doi.org/10.1007/BF00539052

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