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Rhodopsin and other proteins in artificial lipid membranes

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Abstract

Some basic aspects of incorporation of hydrophobic peptides and proteins in artificial lipid membranes are discussed. As examples valinomycin as a carrier model and gramicidin A as a channel former in lipid vesicles and in planar lipid membranes are presented.

In the second part of the lecture some examples of incorporation of membrane proteins into lipid vesicles and planar lipid membranes are reported. The interaction with artificial lipid membranes of the Ca+ ATPase from the sarcoplasmic reticulum, of Rhodopsin, and of Bacteriorhodopsin is presented.

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References

  1. Hsu, M., Chan, S. I.: Nuclear magnetic resonance studies of the interaction of valinomycin with unsonicated lecithin bilayers. Biochemistry 13, 3872–3876 (1973)

    Google Scholar 

  2. Stark, G., Ketterer, B., Benz, R., LÄuger, P.: The rate constants of valinomycin-mediated ion transport through thin lipid membranes. Biophys. J. 11, 981–994 (1971)

    Google Scholar 

  3. Weidekamm, E., Bamberg, E., Brdiczka, D., Wildermuth, G., Macco, F., Lehmann, W., Weber, R.: Raman spectroscopic investigation of the interaction of gramicidin A with dipalmitoyl phosphatidylcholine liposomes. Biochim. Biophys. Acta (Amst.) 464, 442–447 (1977)

    Google Scholar 

  4. Hladky, S. B., Haydon, D. A.: Discreteness of conductance changes in bimolecular lipid membranes in the presence of certain antibiotics. Nature (Lond.) 225, 451 (1970)

    Google Scholar 

  5. Urry, D. W.:The gramicidin A transmembrane channel: A prosposed π (L,D) helix. Proc. Natl. Acad. Sci. USA 68, 672–676 (1971)

    Google Scholar 

  6. Veatch, W. R., Mathies, R., Eisenberg, M., Stryer, L.: Simultaneous fluorescence and conductance studies of planar bilayer membranes containing a highly active and fluorescent analog of gramicidin A. J. Mol. Biol. 99, 75–92 (1975)

    Google Scholar 

  7. Apell, H.-J., Bamberg, E., Alpes, H., LÄuger, P.: Formation of ion channels by a negatively charged analog of gramicidin A. J. Membrane Biol. (in press) (1976)

  8. Warren, G. B., Toon, P. A., Birdsall, N. J. M., Lee, A. G., Metcalfe, J. C.: Reconstitution of a calcium pump using defined membrane components. Proc. Natl. Acad. Sci. USA 71, 622–626 (1974)

    Google Scholar 

  9. Repke, D. I., Spivak, J. C., Katz, A. M.: Reconstitution of an active calcium pump in sarcoplasmic reticulum. J. Biol. Chem. 24, 3169–3175 (1976)

    Google Scholar 

  10. Shamoo, A. E., McLennan, D. H.: A Ca++-dependent and -selective ionophore as part of the Ca++ + Mg++-dependent adenosinetriphosphatase of sarcoplasmic reticulum. Proc. Natl. Acad. Sci. USA 71, 3522–3526 (1974)

    Google Scholar 

  11. Shamoo, A. E., Ryan, T. E.: Localization of ionophore activity in a 20,000-dalton fragment of the adenosine triphosphatase of sarcoplasmic reticulum. J. Biol. Chem. 251, 4147–4154 (1976)

    Google Scholar 

  12. Hong, K., Hubbell, W. L.: Preparation and properties of phospholipid bilayers containing rhodopsin. Proc. Natl. Acad. Sci. USA 69, 2617–2621 (1972)

    Google Scholar 

  13. Darszon, A., Montal, M.: Light increases the ion permeability of rhodopsin-phospholipid bilayer vesicles. In: Mitochondria — Biogenesis — Bioenergetics and Membrane Structure. London: Academic Press 1976

    Google Scholar 

  14. Montal, M.: Rhodopsin in experimental membranes: An approach to elucidate its role in the process of phototransduction. In: Molecular Aspects of Membrane Phenomena. Berlin-Heidelberg-New York: Springer 1975

    Google Scholar 

  15. Racker, E., Stoeckenius, W.: Reconstitution of purple membrane vesicles catalyzing light-driven proton uptake and adenosine triphosphate formation. J. Biol. Chem. 249, 662–663 (1974)

    Google Scholar 

  16. Racker, E., Hinkle, P. C.: Effect of temperature on the function of a proton pump. J. Membrane Biol. 17, 181–188 (1974)

    Google Scholar 

  17. Drachev, L. A., Jasaitis, A. A., Kaulen, A. D., Kondrashin, A. A., Liberman, E. A., Nemececk, I. B., Ostroumov, S. A., Semenov, A. Yu., Skulachev, V. P.: Direct measurement of electric current generation by cytochrome oxidase, H-ATPase and bacteriorhodopsin. Nature (Lond.) 249, 321–324 (1974)s

    Google Scholar 

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  1. Department of Biology, University of Konstanz, Postfach 7733, D-7750, Konstanz, Federal Republic of Germany

    E. Bamberg

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  1. E. Bamberg
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Bamberg, E. Rhodopsin and other proteins in artificial lipid membranes. Biophys. Struct. Mechanism 3, 39–42 (1977). https://doi.org/10.1007/BF00536452

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  • DOI: https://doi.org/10.1007/BF00536452

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