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Affinity chromatography of α,α-trehalase: Coupling of oligosaccharides to aminohexyl sepharose

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Summary

Reducing oligosaccharides were covalently linked to the spacer arm of aminohexyl Sepharose by reductive amination. The reaction was carried out in the presence of sodium borohydride in aqueous medium under mild experimental conditions. An affinity column containing lactose-coupled Sepharose was used for the purification of α,α-trehalase from Artemia salina embryos.

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Authors and Affiliations

  1. Institute of General Physiology, Chair of Molecular Biology, University of Rome and Laboratory of Molecular Embryology, Arco Felice, I-80072, Napoli, Italy

    Mario Bergami & Marcello G. Cacace

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  1. Mario Bergami
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  2. Marcello G. Cacace
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Bergami, M., Cacace, M.G. Affinity chromatography of α,α-trehalase: Coupling of oligosaccharides to aminohexyl sepharose. European J. Appl. Microbiol. Biotechnol. 7, 53–57 (1979). https://doi.org/10.1007/BF00522478

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  • DOI: https://doi.org/10.1007/BF00522478

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