Summary
Below 21° C isolated hepatocytes are insensitive to phalloidin. Between 21 and 30° C the amount of affected cells increases to nearly maximal values, whereas a further rise of the temperature has little additional effect. These findings suggest that phase transition of membranal lipids might be responsible for the unusual temperature characteristics. The fluidity of lipids seems to control an early step of the phalloidin response.
This is a preview of subscription content, log in via an institution to check access.
References
Berry, M. N., Friend, D. S.: High-yield preparation of isolated rat liver parenchymal cells. J. Cell Biol. 43, 506–520 (1969)
Frimmer, M.: Temperature dependence of potassium depletion in the phalloidin poisoned perfused rat liver. Naunyn-Schmiedeberg's Arch Pharmacol. 272, 354–357 (1972)
Frimmer, M.: The role of plasma membranes in hepatotoxic effects. Pharmacology Meeting, Hannover 1976, Naunyn-Schmiedeberg's Arch. Pharmacol. 297, S15-S19 (1977)
Frimmer, M., Kroker, R., Porstendörfer, J.: The mode of action of phalloidin: Demonstration of rapid deformation of isolated hepatocytes by scanning electron microscopy. Naunyn-Schmiedeberg's Arch. Pharmacol. 284, 395–398 (1974)
Frimmer, M., Petzinger, E., Homann, J.: Phalloidin-Antagonisten. 4. Mitteilung: Thioctsäure, SH-Verbindungen, Rifampicin, Choleretika, Dexamethason, Östradiol, unspezifische Hemmstoffe und unwirksame Verbindungen. Arzneim.-Forsch. (Drug Res.) 25, 1881–1884 (1975)
Frimmer, M., Petzinger, E., Rufeger, U., Viel, L. B.: Trypsin protection of hepatocytes against phalloidin. Naunyn-Schmiedeberg's Arch. Pharmacol. 300, 163–171 (1977)
Petzinger, E.: Die Vergiftung isolierter Rattenhepatozyten mit Phalloidin und deren Beeinflussung durch Trypsin und Phalloidinantagonisten. Vet.-Med. Diss., Gießen 1975
Petzinger, E., Seeger, R.: Scanning electron microscopic studies on the cytolytic effect of phallolysin on isolated rat hepatocytes and AS-30 D hepatoma cells. Naunyn-Schmiedeberg's Arch. Pharmacol. 295, 211–213 (1976)
Petzinger, E., Homann, J., Frimmer, M.: Phalloidin-Antagonisten. 2. Mitteilung: Protektive Wirkung von Disilybin bei der Vergiftung isolierter Hepatozyten mit Phalloidin. Arzneim.-Forsch. (Drug Res.) 25, 571–576 (1975)
Petzinger, E., Rufeger, U., Frimmer, M.: Transient desensibilization of isolated hepatocytes against phalloidin by treatment with phospholipase A. Naunyn-Schmiedeberg's Arch. Pharmacol. (in press, 1977)
Puchinger, H.: Zum Wirkungsmechanismus von Phalloidin und Antamanid. Inaug.-Diss., Frankfurt/M. 1968 (Naturwiss. Fak.)
Weiss, E., Sterz, I., Frimmer, M., Kroker, R.: Electron microscopy of isolated rat hepatocytes before and after treatment with phalloidin. Beitr. Path. 150, 345–356 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Frimmer, M., Rufeger, U. Temperature dependence of phalloidin response in isolated rat hepatocytes. Naunyn-Schmiedeberg's Arch. Pharmacol. 300, 193–194 (1977). https://doi.org/10.1007/BF00505051
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00505051