Summary
0.05 ml plasma samples are incubated with 3H-S-adenosylmethionine and catechol-O-methyl-transferase. The resulting methoxy derivatives are extracted, the extracts separated by high pressure liquid chromatography and the metanephrine fractions collected. Evaluation is performed by liquid scintillation counting of radioactivity in the respective fractions. The following performance criteria are presented: precision, accuracy, detectability (40 pg/ml for adrenaline and noradrenaline, 130 pg/ml for dopamine), linearity and day-by-day variation. Comparison with a standard method shows an excellent correlation for adrenaline and noradrenaline.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Axelrod J, Tomchick R (1958) Enzymatic O-methylation of epinephrine and other catechols. J Biol Chem 233:702–705
Büttner J, Borth R, Boutwell JH, Broughton PMG, Bowyer RC (1980) Approved recommendation (1978) on quality control in clinical chemistry. J Clin Chem Clin Biochem 18:69–77
Cryer EP, Santiago JV, Shah S (1974) Measurement of norepinephrine and epinephrine in small volumes of human plasma by a single isotope derivative method: response to the upright posture. Clin Endocrinol Metab 39:1025–1029
Da Prada M, Zürcher G (1976) Simultaneous radioenzymatic determination of plasma and tissue adrenaline, noradrenaline and dopamine within the femtomole range. Life Sci 19:1161–1174
Demeny M, Kirschner AK, Naftchi NE (1979) A new sensitive radioenzymatic-high pressure liquid chromatographic method for the analysis of plasma catecholamines. In: Usdin E, Kopin IJ, Barchas J (eds) Catecholamines: Basic and clinical frontiers, vol 1. Pergamon Press, New York, pp 862–864
Dimsdale JE, Moss J (1980) Plasma catecholamines in stress and exercise. JAMA 243:340–342
Erikson B-M, Andersson I, Borg KO, Persson BA (1977) Determination of adrenaline and noradrenaline in plasma by an isotope derivate method and ion pair liquid chromatography. Acta Pharm Suec 14:451–458
Guldberg HC, Marsden CA (1975) Catechol-O-methyl transferase: Pharmacological aspects and physiological role. Pharmacol Rev 27:135–206
Hansson C, Edholm L-E, Agrup G, Rorsman H, Rosengren A-M, Rosengren E (1978) The quantitative determination of 5-S-cysteinyldopa and dopa in normal serum and in serum from patients with malignant melanoma by means of high-pressure liquid chromatography. Clin Chim Acta 88:419–427
Henry DP, Starman BJ, Johnson DG, Williams RH (1975) A sensitive radioenzymatic assay for norepinephrine in tissue and plasma. Life Sci 16:375–384
Hörtnagel H, Benedict CR, Grahame-Smith DG, McGrath B (1977) A sensitive radioenzymatic assay for adrenaline and noradrenaline in plasma. Br J Clin Pharmacol 4:553–558
Hjemdahl P, Daleskog M, Kahan T (1979) Determination of plasma catecholamines by high performance liquid chromatography with electrochemical detection: Comparison with a radioenzymatic method. Life Sci 25:131–138
Imai K, Tamura Z (1978) Liquid chromatographic determination of urinary dopamine and norepinephrine as fluorescamine derivatives. Clin Chim Acta 85:1–6
Kissinger PT, Riggin RM, Alcorn RL, Rau Leh-Daw (1975) Estimation of catecholamines in urine by high performance liquid chromatography with electrochemical detection. Biochem Med 13:299–306
Klaniecki TS, Corder CN, McDonald, RH, Jr, Feldman JA (1977) High-performance liquid chromatographic radioenzymatic assay for plasma catecholamines. J Lab Clin Med 90:604–612
Kopin IJ, Lake RC, Ziegler MG (1978) Plasma levels of norepinephrine. Ann Int Med 88:671–680
Lake CR, Ziegler MG, Kopin IJ (1976) Use of plasma norepinephrine for evaluation of sympathetic neuronal function in man. Life Sci 18:1315–1326
Mell LD, Gustafson AB (1977) Urinary free norepinephrine and dopamine determined by reverse-phase high-pressure liquid chromatography. Clin Chem 23:473–476
Müller Th, Hofschuster E, Kuss H-J, Welter D (1979) A highly sensitive and precise radioenzymatic assay for plasma epinephrine and norepinephrine. J Neural Transmission 45:219–225
Passon PG, Peuler JD (1973) A simplified radiometric assay for plasma norepinephrine and epinephrine. Analyt Biochem 51:618–631
Peuler JD, Johnson GA (1977) Simultaneous single isotope radioenzymatic assay of plasma norepinephrine, epinephrine and dopamine. Life Sci 21:625–636
Refshauge C, Kissinger PT, Dreiling R, Blank LR, Freeman R, Adams RN (1974) New high performance liquid chromatographic analysis of brain catecholamines. Life Sci 14:311–322
Sole MJ, Hussain MN (1977) A simple radioenzymatic assay for the simultaneous measurement of picogram quantities of norepinephrine, epinephrine, and dopamine in plasma and tissues. Biochem Med 18:301–307
Uihlein M, Appel E, Palm D, Hajdú P, Staib H (1978) Determination of plasma catecholamines by means of radioenzymatic labelling and HPLC separation. Abstracts Euroanalysis III, Dublin, p 351
Watson RDS, Hamilton CA, Reid JL, Littler WA (1979) Changes in plasma norepinephrine, blood pressure and heart rate during physical activity in hypertensive man. Hypertension 1:341–346
Author information
Authors and Affiliations
Additional information
Dedicated to Professor Dr. Herbert Oelschläger for his 60th birthday
Rights and permissions
About this article
Cite this article
Appel, E., Bayer, P., Hajdú, P. et al. Determination of plasma catecholamines by means of radioenzymatic labelling and high pressure liquid chromatographic separation. Naunyn-Schmiedeberg's Arch. Pharmacol. 315, 233–239 (1981). https://doi.org/10.1007/BF00499840
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00499840