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Characterization of alcohol dehydrogenase in young soybean seedlings

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Abstract

Molecular properties of alcohol dehydrogenase (ADH) were examined in young soybean seedlings. Soybean radicle tissue is ADH-rich. Enzyme specific activity decreases slowly with the development of roots and becomes almost undetectable when the first true leaves appear. Soybean ADH was not found to be inducible by flooding. 2,4-Dichlorophenoxyacetic acid (2,4-D) treatment increased ADH specific activity as much as 14-fold. Only one ADH isozyme was detected by isoelectric focusing. By DNA-DNA hybridization, soybean ADH genomic sequences were shown to be partly homologous to maize ADH1 cDNA. The presence of more than one Adh gene in soybean is discussed.

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Author notes

  1. Ryszard Brzezinski

    Present address: Départment de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500, boul. de l'Université, J1K 2R1, Sherbrooke, Québec, Canada

  2. Douglas Brown

    Present address: Department of Biology, Bishop's University, J1M 1Z7, Lennoxville, Québec, Canada

Authors and Affiliations

  1. Départment de Microbiologie, Faculté de Médicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Québec, Canada

    Ryszard Brzezinski, Danuta Klimuszko, Stephen D. Blakeley & Jean-Paul Thirion

  2. Départment de Biochimie, Faculté de Médicine, Université de Sherbrooke, J1H 5N4, Sherbrooke, Québec, Canada

    Brian G. Talbot

Authors
  1. Ryszard Brzezinski
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  2. Brian G. Talbot
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  3. Douglas Brown
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  4. Danuta Klimuszko
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  5. Stephen D. Blakeley
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  6. Jean-Paul Thirion
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This work was supported by a grant from the Medical Research Council of Canada.

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Brzezinski, R., Talbot, B.G., Brown, D. et al. Characterization of alcohol dehydrogenase in young soybean seedlings. Biochem Genet 24, 643–656 (1986). https://doi.org/10.1007/BF00498999

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  • DOI: https://doi.org/10.1007/BF00498999

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