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A technical note on the histochemical demonstration of G6Pase activity

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Summary

Difficulties in the histochemical demonstration of even maximum G6Pase activity can be overcome when unfixed cryostat sections are immersed in a medium which is shaken during incubation. The medium contains: 20 mM G6P, 4.6 mM Pb(NO3)2, 50 mM tris-maleate buffer, pH 6.5.

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References

  • Chiquoine AD (1953) The distribution of glucose-6-phosphatase in the liver and kidney of the mouse. J Histochem Cytochem 1:429–439

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  • Wachstein M, Meisel E (1956) On the histochemical demonstration of glucose-6-phosphatase. J Histochem Cytochem 4:592

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Authors and Affiliations

  1. Anatomisches Institut der Universität Basel, Pestalozzistrasse 20, CH-4056, Basel, Switzerland

    I. P. Maly & D. Sasse

Authors
  1. I. P. Maly
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  2. D. Sasse
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Supported by grants from the Deutsche Forschungsgemeinschaft (Sa 127/8)

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Maly, I.P., Sasse, D. A technical note on the histochemical demonstration of G6Pase activity. Histochemistry 78, 409–411 (1983). https://doi.org/10.1007/BF00496628

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  • DOI: https://doi.org/10.1007/BF00496628

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