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The drug metabolism systems of liver and liver tumors: A comparison of activities and characteristics

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Summary

Transplantable rat liver tumors 5123 t.c., 7288 ct.c., 5123 t.c.(H) and the Novikoff hepatoma have active mixed function oxidase systems capable of metabolizing a variety of drug and polycyclic hydrocarbon substrates. The tumor drug metabolism systems are at best 20% as active as rat liver. The tumor drug metabolism activities are induced by pretreatment with phenobarbital or β-naphthoflavone and can be inhibited with specific inhibitors such as carbon monoxide or 7,8-benzoflavone. Tumor drug metabolism systems appear to consist of cytochrome P-450 and cytochrome P-450 reductase. The properties of the two protein components from tumors are highly similar to the corresponding components of the liver drug metabolism system.

Cytochrome P-450 reductase has been at least partially purified from the Novikoff hepatoma and hepatoma 5123 t.c.(H). The kinetic and physical properties of the tumor reductases are similar to those of the liver reductase except that the Km of hepatoma 5123 t.c.(H)

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  1. John D. Dignam & Susan E. Saine

    Present address: Department of Biochemistry University of Connecticut, Health Center Farmington, 06032, Connecticut, USA

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston, P. O. Box 20708, 77025, Houston, Texas, USA

    Henry W. Strobel, John D. Dignam, Susan E. Saine, Wan-Fen Fang & Pearlie M. Fennell

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Strobel, H.W., Dignam, J.D., Saine, S.E. et al. The drug metabolism systems of liver and liver tumors: A comparison of activities and characteristics. Mol Cell Biochem 22, 79–92 (1978). https://doi.org/10.1007/BF00496236

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