Summary
The elemental composition of the human atheroselerotic popliteal artery was examined using the proton-induced X-ray-emission (PIXE) method. The application of a narrow proton beam (3×10 μm2) enabled us to determine not only the concentrations of Cl, K, Ca, Fe, Cu, Zn, Br and Pb, but also their localization in different artery-wall regions. The highest mean concentrations of Cl, K, Zn and Br were found in the tunica media. In the investigated sections the distribution of Ca and Fe varied: sometimes, these elements were prevalent in the tunica intima, whereas in other cases, the highest concentrations were observed in the tunica media or tunica adventitia. The concentration profiles of each element were characterized by many sharp, narrow peaks. The highest concentrations of Ca and Fe showed such high levels that only one explanation is possible, i.e. the presence of crystals. The correlation of Ca peaks with those of Zn and Fe is discussed. The usefulness of the micro-PIXE method for the investigation of biomedical materials is also considered.
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References
Brocker W, Hohling HJ, Nicholson WAP, Krefting ER, Schreiber J, Schlake W, Druen B (1978) Comparison of the methods of cathodoluminescence, electronprobe microanalysis, and calcium staining, applied to human aorta with isthmus stenosis. Pathol Res Pract 163:310–322
Cichocki T, Glazur J, Jarczyk L, Rokita E, Strzałkowski A, Sych M (1984) Comparison of global elemental composition of liver tissue with composition of different cell fractions. Nucl Instrum Methods B 3:618–620
Demortier G (ed) (1982) Proceedings of the 2nd International Conference on Chemical Analysis. Namur, Belgium, September 8–10, 1981. Nucl Instrum Methods 197: -
Erasmus DA (1978) Electron probe microanalysis in biology. Chapman and Hall, London
Glimcher MJ (1976) Composition, structure, and organization of bone and other mineralized tissue and the mechanism of calcification. In: Greep RO, Astwood ED (eds) Handbook of physiology, vol. 7: Endocrinology. American Physiological Society, Washington DC, pp 25–116
Heck D (1979) The Karlsruhe proton microbeam system. Beitr. Elektronenmikroskop Direktabb. Oberfl 12/1:259–262
Heck D, Rokita E (1983) Elemental analysis performed with the Karlsruhe Nuclear Microprobe. IEEE Trans NS-30:1220–1223
Heck D, Rokita E (1984) Local matrix mass thickness determination in scanned micropixe by proton backscattering. Nucl Instrum Methods B3:259–262
Johansson SAE (ed) (1977) Proceedings of the 1st International Conference on PIXE and its Analytical Applications, Lund, Sweden, August 23–26, 1976. Nucl Instrum Methods 142
Johansson SAE (ed) (1981) Proceedings of the 2nd International Conference on PIXE and its Analytical Applications, Lund, Sweden, June 9–12, 1980. Nucl Instrum Methods 181: -
Krefting ER, Rohrig T, Hohling HJ, Dittrich H, Druen B, Schlake W (1981) Menschliche Aorten mit Isthmusstenose: Elementbestimmungen mit der Elektronenstrahlmikroanalyse. Klin Wochenschr 59:813–815
Morgan AJ, Davies TW, Erasmus DA (1975) Changes in the concentration and distribution of elements during electron microscope preparative procedures. Micron 6:11–23
Pearse AGE (1972) Histochemistry theoretical and applied. 3rd edn, Churchill Livingstone, Edinburgh London
Ross R, Glomset JA (1976) The pathogenesis of atherosclerosis (first of two part). N Engl J Med 295:369–377
Ross R, Glomset JA (1976) The pathogenesis of atherosclerosis (second of two part). N Engl J Med 295:420–425
Schmid K, McSarry WO, Pameijer CH, Binette JP (1980) Chemical and physicochemical studies on the mineral deposits of the human atherosclerotic aorta. Atherosclerosis 37:199–210
Smith EB, Staples EM (1980) Distribution of plasma proteins across the human aortic wall. Atherosclerosis 37:579–590
Underwood EJ (1977) Trace elements in human and animal nutrition. 4th edn. Academic Press, New York, pp 219, 402, 403
Yu SY (1974) Calcification processes in atherosclerosos. In: Wagner WD, Clarkson TB (eds) Arterial mesenchyme an atherosclerosis. Plenum Press, pp 403–425
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Cichocki, T., Heck, D., Jarczyk, L. et al. Elemental composition of the human atherosclerotic artery wall. Histochemistry 83, 87–92 (1985). https://doi.org/10.1007/BF00495306
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DOI: https://doi.org/10.1007/BF00495306