Skip to main content
SpringerLink
Log in

Separation of sugars by HPLC with electrochemical detection

Trennung von Zuckern durch HPLC mit elektrochemischer Detektion

  • Original Papers
  • Natural Substances
  • Published:
Fresenius' Zeitschrift für analytische Chemie Aims and scope Submit manuscript

Zusammenfassung

Da für die Bestimmung von Zuckern nach HPLC-Trennung ein Bedarf an neuen empfindlichen Detektoren besteht, wurde das voltammetrische Verhalten einiger Mono- und Disaccharide untersucht. Nickel-Elektroden erwiesen sich als gut geeignet für dieses Problem. Die Eigenschaften der Elektrode wurden in einem stationÄren System, im flie\enden System und in Kopplung mit der Hochdruckflüssigkeitschromatographie untersucht. Parameter für die Optimierung werden diskutiert und die Anwendungen auf Detektion verschiedener Zucker gezeigt.

Summary

In order to develop new sensitive detectors for the determination of sugars after HPLC-separation, the voltammetric behaviour of several mono- and disaccharides has been investigated. Nickel electrodes turned out to be well suited for this problem. The properties of the electrodes were studied in a stationary system, in a flow-injection system and in combination with high-performance liquid chromatography. Parameters for optimisation are discussed and the application to the detection of several sugars is shown.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Heyrovsky J, Smoler I (1932) Collect Czechoslov Chem Commun 4:521

    Google Scholar 

  2. Brunt K (1982) Analyst 107:1261

    Google Scholar 

  3. Schick KG, Magearu VG, Huber CO (1978) Clin Chem 24:448

    Google Scholar 

  4. Morrison TN, Schick KG, Huber CO (1980) Anal Chim Acta 120:75

    Google Scholar 

  5. Hui BS, Huber CO (1982) Anal Chim Acta 134:211

    Google Scholar 

  6. Kafil JB, Huber CO (1982) Anal Chim Acta 139:347

    Google Scholar 

  7. Buchberger W, Winsauer K, Breitwieser C (1982) Fresenius Z Anal Chem 311:517

    Google Scholar 

  8. Fleischmann M, Korinek K, Pletcher D (1971) J Electroanal Chem 31:39

    Google Scholar 

  9. Vertes G, Horanyi G (1974) J Electroanal Chem 52:47

    Google Scholar 

  10. Robertson PM (1980) J Electroanal Chem 111:97

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for Analytical, Organic and Physical Chemistry, Johannes Kepler University Linz, Altenbergerstra\e 69, A-4040, Linz, Austria

    W. Buchberger, K. Winsauer & Ch. Breitwieser

Authors
  1. W. Buchberger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Winsauer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ch. Breitwieser
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Buchberger, W., Winsauer, K. & Breitwieser, C. Separation of sugars by HPLC with electrochemical detection. Z. Anal. Chem. 315, 518–520 (1983). https://doi.org/10.1007/BF00493800

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00493800

Keywords

Search

Navigation