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Pharmacological characterization of the alpha adrenoceptors of the dog basilar artery

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Summary

The effects of alpha adrenoceptor agonists and antagonists on the postsynaptic alpha receptors were examined in the dog basilar, mesenteric and renal arteries and the type of alpha adrenoceptors present was characterized. In the basilar artery, noradrenaline, clonidine and phenylephrine produced almost the same maximal contraction, the pD2 values being 6.51±0.11, 5.49±0.16 and 5.65±0.13, respectively. Yohimbine (1–3×10−8 M) inhibited the contractile responses to noradrenaline and clonidine competitively and the response to phenylephrine noncompetitively. Corynanthine (10−6 M) had no effect on such contractile responses. In the mesenteric and renal arteries, the maximal responses to noradrenaline and phenylephrine were markedly greater than those to clonidine. Yohimbine (10−7–10−5 M) and corynanthine (10−7–10−5 M) both antagonized noradrenaline competitively in these vessels. In the basilar, mesenteric and renal arteries preloaded with 3H-noradrenaline, 3H-efflux induced by electrical transmural stimulation was attenuated by clonidine (10−10–10−7 M), while phenylephrine (10−10–10−8 M) was without effect. Yohimbine at considerably lower concentrations than corynanthine increased the 3H-efflux clicited by electrical stimulation. These results indicate that presynaptic and postsynaptic alpha receptors of the dog basilar artery are largely alpha2 in contrast to those of peripheral arteries.

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References

  • Arunlakshana O, Schild HO (1959) Some quantitative uses of drug antagonists. Br J Pharmacol 14:48–58

    Google Scholar 

  • Berthelsen S, Pettinger WA (1977) A functional basis for classification of α-adrenergic receptors. Life Sci 21:595–606

    Google Scholar 

  • Bevan JA, Duckles SP, Lee TJ-F (1975) Histamine potentiation of nerve-and drug-induced responses of a rabbit cerebral artery. Circ Res 36:647–653

    Google Scholar 

  • Bohr DF, Goulet PL, Taquini AC Jr (1961) Direct tension recording from smooth muscle of resistance vessels from various organs. Angiology 12:478–485

    Google Scholar 

  • Cubeddu LX, Barnes EM, Langer SZ, Weiner N (1974) Release of norepinephrine and dopamine-β-hydroxylase by nerve stimulation. I. Role of neuronal and extraneuronal uptake and of alpha presynaptic receptors. J Pharmacol Exp Ther 190:431–450

    Google Scholar 

  • Dalske HF, Harakal C, Sevy RW, Menkowitz BJ (1974) Catecholamine content and response to norepinephrine of middle cerebral artery. Proc Soc Exp Biol Med 146:718–721

    Google Scholar 

  • Drew GM, Whiting SB (1979) Evidence for two distinct types of postsynaptic α-adrenoceptor in vascular smooth muscle in vivo. Br J Pharmacol 67:207–215

    Google Scholar 

  • Docherty JR, McGrath JC (1980) A comparison of pre- and postjunctional potencies of several alpha-adrenoceptor agonists in the cardiovascular system and anococcygeus muscle of the rat. Naunyn-Schmiedeberg's Arch Pharmacol 312:107–116

    Google Scholar 

  • Dubocovich ML, Langer SZ (1974) Negative feed-back regulation of noradrenaline release by nerve stimulation in the perfused cat's spleen: differences in potency of phenoxybenzamine in blocking the pre- and post-synaptic adrenergic receptors. J Physiol 237:505–519

    Google Scholar 

  • Duckles SP, Bevan JA (1976) Pharmacological characterization of adrenergic receptor of a rabbit cerebral artery in vitro. J Pharmacol Exp Ther 197:371–378

    Google Scholar 

  • Duckles SP, Rapoport R (1979) Release of endogenous norepinephrine from a rabbit cerebral artery. J Pharmacol Exp Ther 211:219–224

    Google Scholar 

  • Edvinsson L, Owman C (1974) Pharmacological characterization of adrenergic alpha and beta receptors mediating the vasomotor responses of cerebral arteries in vitro. Circ Res 35:835–849

    Google Scholar 

  • Fujiwara M, Sakakibara Y, Muramatsu I (1981) Alpha2 receptor and sympathetic transmission in the dog cerebral arteries. Proc. 4th International Symposium on Vascular Neuroeffector Mechanisms. Raven Press (in press)

  • Jarrott B, Louis WJ, Summers RJ (1979) The effect of a series of clonidine analogues on 3H-clonidine binding in rat cerebral cortex. Biochem Pharmacol 27:141–144

    Google Scholar 

  • Jauering RA, Moulds RFW, Shaw J (1978) The action of prazosin in human vascular preparations. Arch Int Pharmacodyn Ther 231:81–89

    Google Scholar 

  • Kobinger W, Pichler L (1980) Relation between cetral sympathoinhibitory and peripheral pre- and postsynaptic α-adrenoceptor as evaluated by different clonidine-like substances in rats. Naunyn-Schmiedeberg's Arch Pharmacol 315:21–27

    Google Scholar 

  • Langer SZ (1974) Presynaptic regulation of catecholamine release. Biochem Pharmacol 23:1973–1800

    Google Scholar 

  • Langer SZ (1977) Presynaptic receptors and their role in the regulation of transmitter release. Br J Pharmacol 60:481–497

    Google Scholar 

  • Langer SZ (1979) Presynaptic adrenoceptor and regulation of release. In: Paton DM (ed) The release of catecholamine from adrenergic neurons. Pergamon Press, Oxford, pp 59–85

    Google Scholar 

  • Langer SZ (1980) Presynaptic regulation of the release of catecholamines. Pharmacol Rev 32:337–362

    Google Scholar 

  • Langer SZ, Massingham R, Shepperson NB (1980) Presence of postsynaptic α2-adrenoceptors of predominantly extrasynaptic location in the vascular smooth muscle of the dog hind limb. Clin Sci 59:225s-228s

    Google Scholar 

  • Langer SZ, Shepperson NB, Massingham R (1981) Preferential noradrenergic innervation of alpha-adrenergic receptors in vascular smooth muscle. Hypertension 3 (Suppl 1): I-112–I-118

    Google Scholar 

  • Lee TJ-F, Su C, Bevan JA (1976) Neurogenic sympathetic vasocontriction of the rabbit basilar artery. Circ Res 39:120–126

    Google Scholar 

  • Muramatsu I, Ikushima S, Fujiwara M (1977) Enhancement by phentolamine of sympathetic contraction of dog basilar artery and α-receptor mediated feedback. In: Owman C, Edvinsson L (eds) Neurogenic control of brain circulation. Pergamon Press, Oxford, pp 192–206

    Google Scholar 

  • Muramatsu I, Fujiwara M, Osumi Y, Shibata S (1978) Vasoconstrictor and dilator actions of nicotine and electrical transmural stimulation on isolated dog cerebral arteries. Blood Vessels 15:110–118

    Google Scholar 

  • Muramatsu I, Fujiwara M, Miura A, Sakakibara Y (1981) Possible involvement of adenine nucleotides in sympathetic neuroeffector mechanisms of dog basilar artery. J Pharmacol Exp Ther 216:401–409

    Google Scholar 

  • Nielsen KC, Owman C (1971) Contractile response and amine receptor mechanisms in isolated middle cerebral artery of the cat. Brain Res 27:33–42

    Google Scholar 

  • Ruffolo RR Jr, Yaden EL, Waddell JE (1980) Receptor interactions of imidazolines. V. Clonidine differentiates postsynaptic alpha adrenergic receptor subtypes in tissues from the rat. J Pharmacol Exp Ther 213:557–561

    Google Scholar 

  • Starke K (1977) Regulation of noradrenaline release by presynaptic receptor systems. Rev Physiol Biochem Pharmacol 77:1–124

    Google Scholar 

  • Starke K (1981) α-Adrenoceptor subclassification. Rev Physiol Biochem Pharmacol 88:199–236

    Google Scholar 

  • Starke K, Endo T, Taube HD (1975) Relative pre- and postsynaptic potencies of α-adrenoceptor agonists in the rabbit pulmonary artery. Naunyn-Schmiedeberg's Arch Pharmacol 291:55–78

    Google Scholar 

  • Starke K, Langer SZ (1979) A note on terminology for presynaptic receptors. In: Langer SZ, Starke K, Dubocovich ML (eds) Presynaptic receptors. Pergamon Press, Oxford, pp 1–3

    Google Scholar 

  • Starke K, Montel H, Gayk W, Merker R (1974) Comparison of the effects of clonidine on pre- and postsynaptic adrenoceptors in the rabbit pulmonary artery. Naunyn-Schmiedeberg's Arch Pharmacol 285:133–150

    Google Scholar 

  • Sullivan AT, Drew GM (1980) Pharmacological characterization of pre- and postsynaptic α-adrenoceptors in dog saphenous vein. Naunyn-Schmiedeberg's Arch Pharmacol 314:249–258

    Google Scholar 

  • Timmermans PBMWM, Kwa HY, van Zwieten PA (1979) Possible subdivision of postsynaptic α-adrenoceptors mediating pressor responses in the pithed rat. Naunyn-Schmiedeberg's Arch Pharmacol 310:189–193

    Google Scholar 

  • Toda N, Fujita Y (1973) Responsiveness of isolated cerebral and peripheral arteries to serotonin, norepinephrine and transmural electrical stimulation. Circ Res 33:98–104

    Google Scholar 

  • Toda N, Hayashi S, Hattori K (1978) Analysis of the effect of tyramine and norepinephrine in isolated canine cerebral and mesenteric arteries. J Pharmacol Exp Ther 205:382–391

    Google Scholar 

  • U'Prichard DC, Snyder SH (1979) Distinct α-noradrenergic receptors differentiated by binding and physiological relationships. Life Sci 24:78–88

    Google Scholar 

  • Urquilla PR, Marco EJ, Lluch S (1975) Pharmacological receptors of the cerebral arteries of the goat. Blood Vessels 12:53–67

    Google Scholar 

  • Weitzell R, Tanaka T, Starke K (1979) Pre- and postsynaptic effects of yohimbine steroisomers on noradrenergic transmission in the pulmonary artery of the rabbit. Naunyn-Schmiedeberg's Arch Pharmacol 308:127–136

    Google Scholar 

  • Westfall TC (1977) Local regulation of adrenergic neurotransmission. Physiol Rev 57:659–728

    Google Scholar 

  • Wikberg JES (1978) Pharmacological classification of adrenergic α-receptors in the guinea pig. Nature (Lond) 237:164–166

    Google Scholar 

Download references

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This work was supported by a Grant-in-Aid for Co-operative Research (No. 00437006), by a Grant-in-Aid for Special Project Research (No. 56220016) and by a Grant-in-Aid for Encouragement of Young Scientists (No. 577106, 56770118) from the Ministry of Education, Science and Culture, Japan

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Sakakibara, Y., Fujiwara, M. & Muramatsu, I. Pharmacological characterization of the alpha adrenoceptors of the dog basilar artery. Naunyn-Schmiedeberg's Arch. Pharmacol. 319, 1–7 (1982). https://doi.org/10.1007/BF00491469

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  • DOI: https://doi.org/10.1007/BF00491469

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