Summary
A direct immunohistochemical method of high specificity is presented for the demonstration of sites of cathepsin B1. Antisera were raised in rabbits against pure enzyme preparations. The specific antibodies were subsequently isolated and labelled according to methods previously published. Sections from tissues specially rich in this enzyme like liver, kidney, intestinal epithelia and the active macrophages of talcum granulomas were studied. The results suggest a localization of this enzyme mainly to lysosomal organelles and phagocytic vacuoles, which are known from chemical data to contain this enzyme. The prerequisite for a positive tissue reaction is that the enzyme must be present in a form available for antigen-antibody interaction.
Similar content being viewed by others
References
Arnold, W., Mayersbach, H. v.: Changes in the solubility of immunoglobulins after fluorescent labeling and its influence on immunofluorescent techniques. J. Histochem. Cytochem. 20, 975–985 (1972)
Arnon, R., Shapira, E.: Antibodies to papain. A selective fractionation according to inhibitory capacity. Biochemistry 6, 3942–3950 (1967)
Barrett, A. J.: Human cathepsin B1. Purification and some properties of the enzyme. Biochem. J. 131, 809–822 (1973)
Goldman, M.: Fluorescent antibody methods. New York-London: Academic Press 1968
Keilová, H.: On the specificity and inhibition of cathepsins D and B. In: Tissue proteinases, ed. by A. J. Barrett and J. T. Dingle, p. 45–65. Amsterdam: North-Holland Publishing Co. 1971
Niemi, M., Sylvén, B.: On the chemical pathology of interstitial fluid. 2: Intracellular and interstitial enzyme activity in experimental granulomas. Acta path. microbiol. scand. 72, 205–218 (1968)
Otto, K.: Cathepsins B1 and B2. In: Tissue proteinases, ed. by A. J. Barrett and J. T. Dingle, p. 1–28. North-Holland Publishing Co. 1971
Poole, A. R., Dingle, J. T., Barrett, A. J.: The immunocytochemical demonstration of cathepsin D. J. Histochem. Cytochem. 20, 261–265 (1972)
Sjöstrand, F.: Über die Eigenfluoreszenz tierischer Gewebe mit besonderer Berücksichtigung der Säugetierniere. Stockholm: P. A. Norstedt & Söner 1944
Snellman, O.: Cathepsin B, the lysosomal thiol proteinase of calf liver. Biochem. J. 114, 673–678 (1969)
Snellman, O., Sylvén, B.: Antibodies against the lysosomal cathepsin B1. Experientia (Basel) to be published 1974.
Sylvén, B.: Studies on the histochemical “Leucine Aminopeptidase” reaction. VI. The selective demonstration of cathepsin B activity by means of the naphthylamide reaction. Histochemie 15, 150–159 (1968)
Sylvén, B., Snellman, O.: Studies on the histochemical “Leucine Aminopeptidase” reaction. V. Cathepsin B as a potential effector of LNA hydrolysis. Histochemie 12, 240–243 (1968)
Weston, P. D.: A specific antiserum to lysosomal cathepsin D. Immunology 17, 421–428 (1969)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sylvén, B., Snellman, O. The immunofluorescent demonstration of cathepsin B1 in tissue sections. Histochemistry 38, 35–41 (1974). https://doi.org/10.1007/BF00490218
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00490218