Summary
Enkephalin (ENK)- and neurotensin (NT)-immunoreactivities (IR) were localized in cat adrenal medulla using immunocytochemistry. ENK was localized mainly in adrenaline cells, 70%–80% of which were heavily labelled. About 5%–10% of the noradrenaline cells were ENK-immunoreactive (IR). A dense network of ENK-IR nerves was localized among adrenaline cells. NT was localized only in the noradrenaline cells, 60%–70% of which were immunoreactive. NT- and ENK-IR were localized in separate noradrenaline cells. A dense network of NT-IR nerves was restricted among noradrenaline cells.
The present findings confirm that ENK- and NT-like peptides are localized in separate adrenal medullary cells and demonstrate that also the preganglionic nerves are divided in subpopulations according to their neuropeptide content. These nerves have a selective localization among adrenaline and noradrenaline cells and may have physiological significance in the control of secretion of catecholamines and neuropeptides from adrenal medulla.
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References
Anand P, Emson P (1984) Neuropeptides are selective markers of spinal autonomic pathways. TINS 7:268–269
Bendayan M, Zollinger M (1983) Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique. J Histochem Cytochem 31:101–109
Corder R, Mason DFJ, Perrett D, Lowry PJ, Clement-Jones V, Linton EA, Besser GM, Rees LH (1982) Simultaneus release of neurotensin, somatostatin, enkephalins and catecholamines from perfused cat adrenal glands. Neuropeptides 3:9–17
Elde R, Seybold V, Sorenson RL, Cummings S, Holets V, Onstott D, Sasek C, Schmechel DE, Oertel WH (1984) Peptidergic regulation in neuroendocrine and autonomic systems. Peptides 5:101–107
Furness JB, Costa M, Wilson AJ (1977) Water stable fluorophores, produced by reaction with aldehyde solutions, for histochemical localization of catechol- and indolethylamines. Histochemistry 52:159–170
Goedert M, Reynolds GP, Emson PC (1983) Neurotensin in the adrenal medulla. Neurosci Lett 35:155–160
Kobayashi S, Ohashi T, Fujita T, Nakao K, Yoshimasa T, Imura H, Mochizuki T, Yanaihara C, Yanaihara N, Verhofstad AAJ (1983a) An immunohistochemical study on the co-storage of met-enkephalin-arg6-gly7-leu8 and met-enkephalin-arg6-phe7 with adrenaline and/or noradrenaline in the adrenal chromaffin cells of the rat, dog and cat. Biomed Res 4:433–442
Kobayashi S, Ohashi T, Uchida T, Yanaihara N (1983b) Metenkephalin-arg6-gly7-leu8-like immunoreactivity in presynaptic nerve terminals on both adrenaline-storing (A) and noradrenaline-storing (NA) cells of rat adrenal medulla. In: Fujita T, Kanno T, Yanaihara N, Miyoshi A (eds) Brain-gut axis. Biomedical Research Foundation, Tokyo, pp 151–158
Kobayashi S, Ohashi T, Uchida T, Nakao K, Imura H, Yanaihara N, Verhofstad AAJ (1984) Co-storage of adrenaline and noradrenaline with met-enkephalin-arg6-gly7-leu8 and met-enkephalin-arg6-phe7 in chromaffin cells of hamster adrenal medulla. Arch Histol Jpn 47:319–336
Kondo H, Kuramoto H, Wainer BH, Yanaihara N (1985) Evidence for the coexistence of acetylcholine and enkephalin in the sympathetic preganglionic neurons in rats. Brain Res 335:309–314
Krukoff TL, Ciriello J, Calaresu FR (1985) Segmental distribution of peptide-like immunoreactivity in cell bodies of the thoracolumbar sympathetic nuclei of the rat. J Comp Neurol 240:90–102
Livett B, Day R, Elde R, Howe P (1982) Co-storage of enkephalins and adrenaline in the bovine adrenal medulla. Neuroscience 7:1323–1332
Lundberg JM, Rökaeus Å, Hökfelt T, Rosell S, Brown M, Goldstein M (1982a) Neurotensin-like immunoreactivity in the preganglionic sympathetic nerves and in the adrenal medulla of the cat. Acta Physiol Scand 114:153–155
Lundberg J, Hökfelt T, Änggård A, Terenius L, Elde R, Markey K, Goldstein M, Kimmel J (1982b) Organizational principles in the peripheral sympathetic nervous system: Subdivision by coexisting peptides (somatostatin-, avian pancreatic polypeptide- and vasoactive intestinal polypeptide-like immunoreactive materials). Proc Natl Acad Sci USA 79:1303–1307
Miller R, Chang K-J, Cooper B, Cuatrecasas P (1978) Radiimmunoassay and characterization of enkephalins in rat tissues. J Biol Chem 253:531–538
Pelto-Huikko M (1987) Correlative microscopy of catecholamines and neuropeptides in adrenal medulla. In: Hayat M (ed) Correlative microscopy in biology: instrumentation and methods. Academic Press, New York pp 143–155
Pelto-Huikko M, Salminen T, Hervonen A (1982) Enkephalin-like immunoreactivity is restricted to the adrenaline cells in hamster adrenal medulla. Histochemistry 73:493–497
Pelto-Huikko M, Salminen T, Hervonen A (1985a) Localization of enkephalins in adrenaline cells and the nerves innervating adrenaline cells in rat adrenal medulla. Histochemistry 82:377–383
Pelto-Huikko M, Salminen T, Toivanen M, Partanen M, Hervonen A (1985b) Immunohistochemical localization of neurotensin in hamster adrenal medulla. Anat Rec 211:458–464
Reinecke M (1985) Neurotensin: Immunohistochemical localization in central and peripheral nervous system and in endocrine cells and its functional role as neurotransmitter and endocrine hormone. Progress in histochemistry and cytochemistry, vol 16 no. 1. Gustav Fischer, Stuttgart New York
Rökaeus Å, Friend G, Lundberg J (1984) Occurrence, storage and release of neurotensin-like immunoreactivity from the adrenal gland. Acta Physiol Scand 120:373–380
Schultzberg M, Lundberg JM, Hökfelt T, Terenius L, Brandt J, Elde R, Goldstein M (1978) Enkephalin-like immunoreactivity in gland cells and nerve terminals of the adrenal medulla. Neuroscience 3:1169–1186
Terenghi G, Polak JM, Varndell IM, Lee YC, Wharton J, Bloom SR (1983) Neurotensin-like immunoreactivity in a subpopulation of noradrenaline containing cells of the cat adrenal gland. Endocrinology 112:226–233
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Pelto-Huikko, M., Salminen, T. & Hervonen, A. Localization of enkephalin- and neurotensin-like immunoreactivities in cat adrenal medulla. Histochemistry 88, 31–36 (1987). https://doi.org/10.1007/BF00490163
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DOI: https://doi.org/10.1007/BF00490163