Abstract
From polluted water of a lagoon pond a new type of denitrifying photosynthetic purple bacteria was isolated. With respect to morphology, fine structure, photopigments, requirement for growth factors, the range of utilization of organic substrates for phototrophic growth and DNA base ratio, the denitrifying strains show the closest resemblance to Rhodopseudomonas sphaeroides and were therefore described as a subspecies named R. sphaeroides forma sp. denitrificans. The new isolates grow well with nitrate anaerobically in the dark accompanying the evolution of nitrogen gas. They cannot assimilate nitrate as the nitrogen source for growth.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Clayton, R. K.: Absorption spectra of photosynthetic bacteria and their chlorophylls. In: Bacterial photosynthesis (H. Gest, A. San Pietro, L. P. Vernon, eds.), pp. 495–500. Yellow Springs: Antioch Press 1963
Jensen, S. L.: Carotenoids of photosynthetic bacteria—Distribution, structure and biosynthesis. In: Bacterial photosynthesis (H. Gest, A. San Pietro, L. P. Vernon, eds.), pp. 19–34. Yellow Springs: Antioch Press 1963
Katoh, T.: Nitrate reductase in photosynthetic bacterium, Rhodospirillum rubrum. Adaptive formation of nitrate reductase. Plant Cell Physiol. 4, 199–215 (1963)
Ketchum, P. A., Sevilla, C. L.: In vitro formation of nitrate reductase using extracts of the nitrate reductase mutant of Neurospora crassa, nit-1, and Rhodospirillum rubrum. J. Bact. 116, 600–609 (1973)
Kitamura, H.: Studies on purification and utilization of polluted water using photosynthetic bacteria and microalgae (Part 1). Hakko Kyokaishi 30, 76–86 (1972)
Marmur, J.: A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. molec. Biol. 3, 208–218 (1961)
Van Niel, C. B.: The culture, general physiology, morphology and classification of the non-sulfur-purple and brown bacteria. Bact. Rev. 8, 1–118 (1944)
Payne, W. J.: Reduction of nitrogenous oxides by microorganisms. Bact. Rev. 37, 409–452 (1973)
Pfennig, N., Trüper, H. G.: The photosynthetic bacteria. In: Bergey's manual of determinative bacteriology, 8th ed. (R. E. Buchanan, N. E. Gibbons, eds.), pp. 24–75. Baltimore: Williams and Wilkins 1974
Satoh, T., Hoshino, Y., Kitamura, H.: Isolation of denitrifying photosynthetic bacteria. Agr. Biol. Chem. 38, 1749–1751 (1974)
Schildkraut, C., Lifson, S.: Dependence of the melting temperature of DNA on slat concentration. Biopolymers 3, 195–208 (1965)
Schlegel, H. G., Gottschalk, G.: Poly-β-hydroxybuttersäure, ihre Verbreitung, Funktion und Biosynthese. Angew. Chem. 74, 342–347 (1962)
Taniguchi, S., Kamen, M. D.: On the nitrate metabolism of facultative photoheterotrophs. In: Microalgae and photosynthetic bacteria (H. Tamiya, ed.), pp. 465–484. Tokyo: University of Tokyo Press 1963
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Satoh, T., Hoshino, Y. & Kitamura, H. Rhodopseudomonas sphaeroides forma sp. denitrificans, a denitrifying strain as a subspecies of Rhodopseudomonas sphaeroides . Arch. Microbiol. 108, 265–269 (1976). https://doi.org/10.1007/BF00454851
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00454851