Zusammenfassung
Die Aktivitäten von Adenylatzyklase in isolierten cochlearen und vestibulären Strukturen wurden in Inkubationen mit dem ATP-Analog Adenylylimidodiphosphat gemessen. Hohe Aktivitäten (pro mg protein) fanden sich in Stria vascularis und vestibulären Präparationen, geringere Aktivitäten im Ligamentum spirale, dem 8. Nerven und Cortischem Organ. In allen Präparationen wurde die Enzymaktivität stimuliert durch Fluorid, Guanylylimidodiphosphat oder Mangan(II)ionen und gehemmt durch Ethakrynsäure und Bleiionen. Das ototoxische Zytostatikum cis-Diammin-dichloroplatin hemmte die Zyklase von Stria vascularis signifikant.
Summary
Adenylate cyclase activity of dissected cochlear and vestibular structures was assayed with the ATP-analog adenylyl imidodiphosphate as substrate. High activities (per mg protein) were found in stria vascularis and in vestibular preparations, lower activities in spiral ligament, VIIIth nerve, and organ of Corti. The enzyme from all structures was stimulated by fluoride, guanylyl imidodiphosphate or manganese(II) ions, and strongly inhibited by ethacrynate and lead ion. The anti-cancer drug cis-diammine-dichloro platinum significantly inhibited adenylate cyclase from stria vascularis.
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This work was supported by grant NS 12881 from the National Institutes of Health
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Bagger-Sjöbäck, D., Filipek, C.S. & Schacht, J. Characteristics and drug responses of cochlear and vestibular adenylate cyclase. Arch Otorhinolaryngol 228, 217–222 (1980). https://doi.org/10.1007/BF00454231
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DOI: https://doi.org/10.1007/BF00454231