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Molecular cloning of a beta-glucosidase-encoding gene from Sclerotinia sclerotiorum by expression in Escherichia coli

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Summary

Six Sclerotinia sclerotiorum genes previously described as being expressed specifically during the induction of cell-wall-degrading enzymes have been transferred to Escherichia coli. When tested for expression of beta-glucosidase activity using X-glu (5-bromo-4-chloro-3-indolyl-beta-d-glucopyranoside), one of of the the genes expressed a X-glu-degrading activity. The corresponding RNA size is reported.

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Authors and Affiliations

  1. Department of Microbiology, The Medical School, University Walk, BS8 1TD, Bristol, UK

    Gabriel Waksman

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  1. Gabriel Waksman
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Waksman, G. Molecular cloning of a beta-glucosidase-encoding gene from Sclerotinia sclerotiorum by expression in Escherichia coli . Curr Genet 15, 295–297 (1989). https://doi.org/10.1007/BF00447047

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  • DOI: https://doi.org/10.1007/BF00447047

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