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Defects in cytochrome cd 1-dependent nitrite respiration of transposon Tn5-induced mutants from Pseudomonas stutzeri

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Abstract

Mutants with defective respiratory nitrite utilization (Nir- phenotype) were obtained by transposon Tn5 insertion into genomic DNA of the ZoBell strain of Pseudomonas stutzeri. Three representative mutants were characterized with respect to their activities of nitrite and nitric oxide reduction, cytochrome cd 1 content, and pattern of soluble c-type cytochromes. Mutant strain MK201 over-produced cytochrome c 552 about fourfold by comparison with the wild type, but possessed an in vitro functional cytochrome cd 1. Mutant strain MK202 lacked cytochrome cd 1 and, simultaneously, had low amounts of cytochrome c 552 and the split α-peak c-type cytochrome. Strain MK203 synthesized nitrite reductase defective in the heme d 1 prosthetic group. Irrespective of these biochemically distinct Nir- phenotypes, all mutants preserved the nitric oxidereducing capability of the wild type. The mutant characteristics demonstrate that cytochrome cd 1 is essential for nitrite respiration of P. stutzeri and establish the presence of a nitric oxide-reducing system distinct from cytochrome cd 1. They also indicate the functional or regulatory interdependence of c-type cytochromes.

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Author notes

  1. K. Döhler

    Present address: Lehrstuhl für Genetik, Universität Bayreuth, D-8580, Bayreuth

  2. K. Frunzke

    Present address: Lehrstuhl für Mikrobiologie, Universität Bayreuth, D-8580, Bayreuth

Authors and Affiliations

  1. Lehrstuhl für Mikrobiologie der Universität Karlsruhe, Kaiserstrasse 12, D-7500, Karlsruhe 1, Germany

    W. G. Zumft, K. Döhler, H. Körner, S. Löchelt, A. Viebrock & K. Frunzke

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  1. W. G. Zumft
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  2. K. Döhler
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  3. H. Körner
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  4. S. Löchelt
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  5. A. Viebrock
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  6. K. Frunzke
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Zumft, W.G., Döhler, K., Körner, H. et al. Defects in cytochrome cd 1-dependent nitrite respiration of transposon Tn5-induced mutants from Pseudomonas stutzeri . Arch. Microbiol. 149, 492–498 (1988). https://doi.org/10.1007/BF00446750

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  • DOI: https://doi.org/10.1007/BF00446750

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