Abstract
Eleven-kilogram parcels of 2-row and 6-row barley initially at 18% moisture content were implanted in dry bulk oats in a farm granary in Manitoba for 60 weeks between August 1983 and October 1984. Temperature, moisture content, O2 and CO2 levels, fat acidity values, seed germination, microfloral incidence and abundance and the presence of major mycotoxins (aflatoxins, sterigmatocystin, ochratoxin A, citrinin, penicillinic acid, patulin) were monitored. Ochratoxin A reached maximum levels of 0.97 ppm by week 24 in the 6-row barley, and 0.05 ppm by week 28 in the 2-row; no other mycotoxins were detected. The effect of cultivar type was significant (P<0.01) with greater effects in the 6-row barley for the following parameters: fat acidity value, germination, incidence of Alternaria alternata, Penicillium spp. and Helminthosporium sativum, total fungal propagule count and ochratoxin A levels. The effect of time was significant (P<0.05) for all variables except oxygen, carbon dioxide, Aspergillus versicolor, and total fungal propagule count. The interaction between cultivar and time was significant (P<0.01) for Alternaria and Helminthosporium only.
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Abramson D, Mills JT: Mycotoxin formation during storage. Chapter 4. In: Sutton MD, Scott PM & Trenholm HL (eds) Mycotoxins: A Canadian Perspective. NRCC Publications, Ottawa, Ontario, 1985.
Abramson D, Mills JT, Boycott BR: Mycotoxins and mycoflora in animal feedstuffs in western Canada. Can J Comp Med 47:23–26, 1983.
Abramson D, Sinha RN, Mills JT: Mycotoxin and odor formation in moist cereal grain during granary storage. Cereal Chem 57:346–351, 1980.
Abramson D, Sinha RN, Mills JT: Mycotoxin and odor formation in barley stored at 16 and 20% moisture in Manitoba. Cereal Chem 60:350–355, 1983.
Abramson D, Sinha RN, Mills JT: Mycotoxin formation and quality changes in granary-stored corn at 16 and 21% moisture content. Sci Alim 5:653–663, 1985.
American Association of Cereal Chemists, 1961. Method 02–01, approved April 1961. Approved Methods of the AACC. American Association of Cerel Chemists, St. Paul, MN, 1962.
American Society of Agricultural Engineers: Moisture measurement — grains and seeds, method S352. Agricultural Engineers' Yearbook. American Society of Agricultural Engineers, St. Joseph, MI, 1975.
Association of Official Analytical Chemists: Official Methods of Analysis, 12th Ed. Association of Official Analytical Chemists, Washington, DC.
Bothast RJ, Rogers RJ, Hesseltine CW: Microbiology of corn and dry-milled corn products. Cereal Chem 51:829–838, 1974.
Hill RA: Barley grain microflora with special reference to Penicillium species. PhD Thesis, University of Reading, Reading, UK, 1979.
Hill RA, Lacey J: Factors determining the microflora of stored barley grain. Ann Appl Biol 102:467–483, 1983.
Josefsson BGE, Moller TE: Screening method for the detection of aflatoxins, ochratoxin, patulin, sterigmatocystin and zearalenone in cereals. J Assoc Off Anal Chem 60:1369–1371, 1977.
Magan N, Lacey J: Effect of temperature and pH on the water relations of field and storage fungi. Trans Br Mycol Soc 82:83–93, 1984.
Mills JT, Abramson D: Ochratoxigenic potential of Penicillium spp. isolated from stored rape seed and cereals in western Canada. Can J Plant Path 4:37–41, 1982.
Mills JT, Abramson D: Production of sterigmatocystin by isolates of Aspergillus versicolor from western Canadian stored barley and rape seed/canola. Can J Pl Path (in press).
Mills JT, Sinha RN, Wallace HAH: Assessment of quality criteria of stored rapeseed — a multivariate study. J Stored Prod Res 14:121–133, 1978.
Muir WE, Waterer D, Sinha RN: Carbon dioxide as an early indicator of stored cereal and oilseed spoilage. Trans Amer Soc Agric Eng 28:1673–1675, 1985.
Neish GA, Cohen H: Vomitoxin and zearalenone production by Fusarium graminearum from winter wheat and barley in Ontario. Can J Plant Sci 61:811–815, 1982.
Puls R, Greenway JA: Fusariotoxicosis from barley in British Columbia. II. Analysis and toxicity of suspected barley. Can J Comp Med 40:16–19, 1976.
Scott PM, van Walbeek W, Kennedy B Anyeti D: Mycotoxins (ochratoxin A, citrinin and sterigmatocysin) and toxigenic fungi in grains and other agricultural products. J Agric Food Chem 20:1103–1109, 1972.
Sinha RH: Interrelations of physical, chemical and biological variables in the deterioration of stored grains. In: Sinha RN, Muir WE (eds), Grain storage — Part of a system. AVI, Westport, 1973, CT:15–47.
Wallace HAH, Sinha RN: Fungi associated with hot-spots in farm-stored grain. Can J Plant Sci 42:48–59, 1962.
Wilson DM, Tabor WH, Trucksess MW: Screening method for the detection of aflatoxin, ochratoxin, zearalenone, penicillic acid and citrinin. J Assoc Off Anal Chem 59:125–127, 1976.
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Contribution No. 1233, Agriculture Canada Research Station, Winnipeg, Manitoba R3T 2M9, Canada.
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Abramson, D., Sinha, R.N. & Mills, J.T. Mycotoxin formation in moist 2-row and 6-row barley during granary storage. Mycopathologia 97, 179–185 (1987). https://doi.org/10.1007/BF00437241
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DOI: https://doi.org/10.1007/BF00437241