Characterization of the gene urf13-T and an unidentified reading frame, ORF 25, in maize and tobacco mitochondria

Summary

We have previously identified two large open reading frames, designated ORF13 and ORF25, in the Texas male-sterile cytoplasm (cms-T) of maize mitochondrial DNA (mtDNA). ORF13 is a single copy gene of chimeric origin that is uniquely transcribed and translated in the mitochondria of cms-T maize, where it produces a polypeptide of approximately 13,000 Mr. The ORF13 reading frame does not occur in the maize N, C or S cytoplasms or Nicotiana tabacum. ORF25 exists as a single copy and is transcribed in the four major maize cytoplasms (N, T, C and S) and N. tabacum. The predicted ORF25 polypeptide has a molecular weight of 24,374 in normal maize and 22,439 in tobacco. Several nucleotide and predicted amino acid changes have occurred in the ORF25 gene among the four maize cytoplasms and N. tabacum. Properties such as transcription and conservation of the sequence between two diverse species suggests that ORF25 encodes a functional plant mitochondrial gene. The ORF25 sequence of maize contains a chloroplast DNA insert homologous to a tRNA-Arg gene; this chloroplast DNA insert is absent in the tobacco ORF25 sequence. Comparison of the ORF25 and ORF13 sequences in restored and non-restored cms-T indicates no differences in their nucleotide sequences. Thus fertility restoration does not alter the primary sequences of ORF13 or ORF25.