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Human skeletal muscle debranching enzyme activities with exercise and training

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Abstract

Sixteen healthy male subjects classified as sedentary (8) or active (8) exercised to exhaustion on a bicycle ergometer at a load requiring approximately 70% of their aerobic capacity. Biopsy samples were taken from the vastus lateralis at rest and at fatigue. A 12 week training program increased skeletal muscle debranching enzyme activities twofold. Submaximal and maximal exercise to exhaustion resulted in decreased debranching enzyme activities. The results indicate that debranching enzyme activities correlate highly with total phosphorylase activities after submaximal and maximal work to fatigue.

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References

  • Huijing, F., Obbink, H. J. K., van Creveld, S.: The activity of the debranching enzyme system in leucocytes: A genetic study of glycogen storage disease Type III. Acta genet. (Basel) 18, 128–136 (1968)

    Google Scholar 

  • Hultman, E.: Studies on muscle metabolism of glycogen and active phosphate in man with special reference to exercise and diet. Scand. J. clin. Lab. Invest. 94, 1–63 (1967)

    Google Scholar 

  • Karlsson, J.: Lactate and phosphagen concentrations in working muscle of man. Acta physiol. scand. Suppl. 358 (1971)

  • Larner, J., Schliselfeld, L. H.: Studies on amylo-1, 6-glucosidase. Biochim. biophys. Acta (Amst.) 20, 53–61 (1956)

    Google Scholar 

  • Piehl, K.: Time course for refilling of glycogen stores in human muscle fibres following exercise-induced glycogen depletion. Acta physiol. scand. (1974) (in press)

  • Smith, E. E.: Enzymic control of glycogen structure. In: Control of glycogen metabolism, pp. 203–213. London: Academic Press 1968

    Google Scholar 

  • Steel, R. G. D., Torrie, J. H.: Principles and procedures of statistics. New York: McGraw Hill 1960

    Google Scholar 

  • Taylor, A. W., Booth, M. A., Rao, S.: Human skeletal muscle phosphorylase activities with exercise and training. Canad. J. physiol. Pharm. 50, 1038–1042 (1972a)

    Google Scholar 

  • Taylor, A. W., Lappage, R., Rao, S.: Skeletal muscle glycogen stores after submaximal and maximal work. Med. Sci. Sports 3, 75–78 (1971)

    Google Scholar 

  • Taylor, A. W., Stothart, J., Booth, M. A., Thayer, R., Rao, S.: Human skeletal muscle glycogen branching enzyme activities with exercise and training. Canad. J. Physiol. Pharm. 529, 119–122 (1974)

    Google Scholar 

  • Taylor, A. W., Thayer, R., Rao, S.: Human skeletal muscle glycogen synthetase activities with exercise and training. Canad. J. physiol. Pharm. 50, 411–415 (1972b)

    Google Scholar 

  • Taylor, P. M., Whelan, W. J.: Rabbit muscle amylo-1, 6-glucosidase: Properties and evidence of heterogeneity. In: Control of glycogen metabolism, pp. 101–114. London: Academic Press 1968

    Google Scholar 

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Supported in part by Grant DNHW 55-04021 from the Department of National Health and Welfare, Ottawa. We thank Miss Diane Tougas and Miss Nancy Smith for technical assistance. This work was completed while A. W. Taylor was under the tenure of a research associateship from the Department of National Health and Welfare, Ottawa. Present address of M. Booth is Departments of Physiology and Physical Education, Queen's University, Kingston, Ontario.

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Taylor, A.W., Stothart, J., Thayer, R. et al. Human skeletal muscle debranching enzyme activities with exercise and training. Europ. J. Appl. Physiol. 33, 327–330 (1974). https://doi.org/10.1007/BF00430240

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  • DOI: https://doi.org/10.1007/BF00430240

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