Summary
Transcriptional complexes formed in vitro using DNA of B. subtilis phage SPP1 as template and E. coli and B. subtilis RNA polymerases were analyzed by electron microscopy. Both enzymes recognize the same five strong promoters in the early region of the genome. Strand selection at these sites was identical with both enzymes. These results correlate well with data obtained from in vivo transcription studies. Transcriptional activity in the late region of the genome was very low, not permitting the identification of promoter sites.
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Amann EP, Reeve JN (1981) Cloning and expression of Bacillus subtilis phage SPP1 in E. coli. II. Expression of λ/SPP1 hybrid phages in E. coli minicells. Mol Gen Genet (in press)
Avila J, Mermoso JM, Vinuela E, Salas M (1971) Prufication and properties of DNA-dependent RNA polymerase from B. subtilis vegetative cells. Eur J Biochem 21:526–535
Behrens B, Lüder G, Behncke M, Trautner TA, Ganesan AT (1979) The genome of B. subtilis phage SPP1. Physical arrangement of phage genes. Mol Gen Genet 175:351–357
Burgess RR, Jendrissak JJ (1975) A procedure for the rapid, large-scale purification of E. coli DNA-dependent RNA polymerase involving polyamin P precipitation and DNA-cellulose chromatography. Biochemistry 14:4634–4638
Chenciner N, Milanesi G (1978) Restriction fragment analysis of bacteriophage SPP1 in vitro transcription by host RNA polymerase. J Virol 28:95–105
Delius H, Westphal H, Axelrod N (1973) Length measurement of RNA synthesized in vitro by Escherichia coli RNA polymerase. J Mol Biol 74:677–687
Esche H, Spatz HC (1973) Asymmetric transcription of SPP1 in vitro. Mol Gen Genet 124:57–63
Esche H (1975) Gene expression of bacteriophage SPP1. II. Regulatory aspects. Mol Gen Genet 142:57–66
Esche H, Schweiger M, Trautner TA (1975) Gene expression of bacteriophage SPP1. I. Phage directed protein synthesis. Mol Gen Genet 142:45–55
Gabain A v, Bujard H (1972) Interaction of E. coli RNA polymerase with promoters of coliphage T5. The rates of complex formation and decay and their correlation with in vitro and in vivo transcriptional activity. Mol Gen Genet 157:301–311
Milanesi G, Cassani G (1972) Transcription after bacteriophage SPP1 infection in B. subtilis. J Virol 10:187–192
Montenegro MA, Esche H, Trautner TA (1976) Induction of mutations in B. subtilis phage SPP1 by growth on host cells carrying a mutator DNA polymerase III. Mol Gen Genet 149:131–134
Montenegro MA, Trautner TA (1981) In vivo transcription of B. subtilis bacteriophage SPP1. Mol Gen Genet 181:512–517
Morelli G, Montenegro MA, Hillenbrand G, Scherzinger E, Trautner TA (1978) The genome of B. subtilis phage SPP1: Assignment of 5′–3′-polarity to the complementary strands of SPP1 DNA. Mol Gen Genet 164:93–97
Morelli G, Fisseau C, Behrens B, Trautner TA, Luh J, Ratcliff SW, Allison DP, Ganesan AT (1979) The genome of B. subtilis phage SPP1: The topology of DNA molecules. Mol Gen Genet 168:153–164
Ratcliff SW, Luh J, Ganesan B, Thompson R, Montenegro MA, Morelli G, Trautner TA (1979) The genome of Bacillus subtilis phage SPP1: The arrangement of restriction endonuclease generated fragments. Mol Gen Genet 168:165–172
Riva SC (1969) Asymmetric transcription of B. subtilis phage SPP1 DNA in vitro. Biochem Biophys Res Commun 34:824–830
Stüber D, Bujard H (1981) The organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc Natl Acad Sci USA (in press)
Stüber D, Delius H, Bujard H (1978) Electron microscopic analysis of in vitro transcriptional complexes: Mapping of promoters of the coliphage T5 genome. Mol Gen Genet 166:141–149
Trautner TA, Spatz HC, Behrens B, Pawlek B, Behncke M (1972) Exchange between complementary strands of DNA? Adv in the Biosciences 8:79–86
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Stüber, D., Morelli, G., Bujard, H. et al. Promoter sites in the genome of B. subtilis phage SPP1. Molec. Gen. Genet. 181, 518–521 (1981). https://doi.org/10.1007/BF00428745
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DOI: https://doi.org/10.1007/BF00428745