Summary
A panel of clones of mink-Chinese hamster somatic cell hybrids was analysed to obtain data for assigning the genes for thymidine kinase-1 (TK1), galactokinase (GALK), subunit C of aldolase (ALDC), and esterase D (ESD) to specific mink chromosomes. The results demonstrate that the genes for TK1, GALK, ALDC and ESD are syntenic and located on mink chromosome 8. Prometaphase analysis of transformed mouse cells obtained by transfer of mink genes by means of metaphase chromosomes demonstrated the presence of mink chromosome 8 fragments of different sizes in some of the independent transformants. Segregation analysis of these fragments and mink TK1, GALK, ALDC and ESD allowed us to assign the genes for TK1 and GALK to 8p24, ALDC to pter-8p25, and ESD to 8q24-8qter.
Similar content being viewed by others
References
Cowmeadow MP, Ruddle FH (1978) Computer-assisted statistical procedures for somatic cell gene assignment. Cytogenet Cell Genet 22:694–697
Gradov AA, Rubtsov NB, Shilov AG, Bochkarev MN, Serov OL (1983) Chromosome localization of the genes for ENO1, HK1, ADK, ACP2, MPI, ITPA, ACON1 and α GAL in the American mink (Mustela vison). Theor Appl Genet 67:59–65
Harris H, Hopkinson DA (1976) Handbook of enzyme electrophoresis in human genetics. North-Holland, Amsterdam
Khlebodarova TM, Serov OL (1980) A new locus regulating the expression of the Ldh-2 gene in mouse liver. Biochem Genet 18:1027–1039
Kit S, Leung W-C, Trkula D (1973a) Distinctive properties of mitochondrial thymidine (dT) kinase from bromodeoxyuridine (dBU)-resistant mouse lines. Biochem Biophys Res Commun 54:455–461
Kit S, Leung W-C, Kaplan LA (1973b) Distinctive molecular forms of thymidine kinase in mitochondria of normal and bromodeoxyuridine-resistant HeLa cells. Eur J Biochem 39:43–48
Kit S, Leung W-C, Trkula D (1974) Gel electrophoresis and isoelectric focusing of mitochondrial and viral-induced thymidine kinases. Int J Cancer 13:203–218
Klobutcher LA, Ruddle FH (1979) Phenotype stabilisation and integration of transferred material in chromosome-mediated gene transfer. Nature 280:657–660
Kozak CA, Ruddle FH (1977) Assignment of the genes for thymidine kinase and galactokinase to Mus musculus chromosome 11, and the preferntial segregation of this chromosome in Chinese hamster/mouse somatic cell hybrids. Somat Cell Genet 3:121–133
McBreen P, Orkwiszewski KG, Chern CJ, Mellman WJ, Croce CM (1977) Synteny of the genes for thymidine kinase and galactokinase in the mouse and their assignment to mouse chromosome 11. Cytogenet Cell Genet 19:7–13
Nichols EA, Elsevier SM, Ruddle FH (1974) A new electrophoretic technique for mouse, human and Chinese hamster galactokinase. Cytogenet Cell Genet 13:275–278
Penhoet E, Kochman M, Rutter WJ (1969) Isolation of fructose diphosphate aldolases A, B, and C. Biochemistry 8:4391–4397
Peterson JL, McBride OW (1980) Cotransfer of linked eukaryotic genes and efficient transfer of hypoxanthine phosphoribosyltransferase by DNA-mediated gene transfer. Proc Natl Acad Sci USA 77:1583–1587
Peterson JL, McBride OW (1983) Close linkage of transferred galactokinase and thymidine kinase genes in a transformant after DNA-mediated gene transfer. In: Genet Eng Eukaryotes, Proc NATO Adv Study Inst, Pullman, Washington 26th July–6th August, 1982. New York, London, pp 53–60
Reznik LG, Reshetnikova GF, Radjabli SI (1980) Estabilishment and chromosome analysis of a new cell line of American mink (Mustela vison). Genetika (in Russian) 16:1020–1025
Rubstov NB, Radjabli SI, Gradov AA, Serov OL (1981a) Chinese hamster x American mink somatic cell hybrids: characterization of a clone panel and assignment of the mink genes for malate dehydrogenase, NADP-1 and malate dehydrogenase, NAD-1. Theor Appl Genet 60:99–106
Rubtsov NB, Radjabli SI, Gradov AA, Serov OL (1981b) Chromosome localization of three syntenic gene pairs in the American mink (Mustela vison). Cytogenet Cell Genet 31:184–187
Rubtsov NB, Radjabli SI, Gradov AA, Serov OL (1982a) Chromosome localization of the genes for isocitrate dehydrogenase-1, isocitrate dehydrogenase-2, glutathione reductase, and phosphoglycerate kinase-1 in the American mink (Mustela vison). Cytogenet Cell Genet 33:256–260
Rubtsov NB, Gradov AA, Serov OL (1982b) Chromosome localization of the loci GOT1, PP, NP, SOD1, PEPA and PEPC in the American mink (Mustela vison). Theor Appl Genet 63:331–336
Serov OL, Bochkarev MN, Khlebodarova TM (1976) Molecular hybridization of native and modified subunits of lactate dehydrogenase isozymes and aldolase A in rats. Biokhimia (in Russian) 41:886–890
Sukoyan MA, Belyaev ND, Budker VG, Gradov AA, Pack SD, Serov OL (1983) Gene transfer by lipid-encapsulated nuclei. Abstr of Joint Congr Eur Tiss Cult Soc (ETCS) and Eur Reticuloend Soc (EURES) 9th–11th May, 1983. Budapest, p 53
Sukoyan MA, Matveeva NM, Belyaev ND, Pack SD, Gradov AA, Shilov AG, Zhdanova NS, Serov OL (1984) Cotransfer and phenotypic stabilisation of syntenic and asyntenic mink genes into mouse cells by chromosome-mediated gene transfer. Mol Gen Genet 196:97–104
Sukoyan MA, Belyaev ND, Budker VG, Gradov AA, Pack SD, Serov OL (1985) Gene transfer into the mouse cells by use of isolated mink lipid-encapsulated nuclei. (in press)
Wijnen LMM, Grzeschik K-H, Pearson PL, Meera Khan P (1977) The human PGM-2 and its chromosomal localization in man-mouse hybrids. Hum Genet 37:271–278
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Gradov, A.A., Pack, S.D., Sukoyan, M.A. et al. Regional assignment of the genes for TK1, GALK, ALDC, and ESD on chromosome 8 in the American mink by chromosome-mediated gene transfer. Molec. Gen. Genet. 200, 433–438 (1985). https://doi.org/10.1007/BF00425727
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00425727