Summary
Fusion complementation experiments between nitrate reductase (NR) deficient lines CNX 20, 27, 82 and 103 of Nicotiana plumbaginifolia were performed with the already characterized N. plumbaginifolia mutants nx 1, 24 and 21, belonging respectively to the complementation groups cnx A, B and C. CNX 20 and 82 were identified as belonging to the group of cnx A. CNX 27 complemented with NX 1 and NX 21 but not with NX 24 indicating another B type. The fourth line, CNX 103 showed complementation with CNX 20, NX 21 and NX 24, revealing a fourth cnx complementation group, cnx D, that until now has not been described in higher plants. Genetic crosses inside respectively the NIA and the CNX group, and between NIA and CNX confirmed the fusion complementation results, and showed allelism for the nia mutants
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References
Cove DJ (1979) Genetic studies of nitrate assimilation in Aspergillus nidulans. Biol Rev 54:291–327
Durand J (1979) High and reproducible plating efficiencies of protoplasts isolated from in vitro grown haploid Nicotiana sylvestris. Z Pflanzenphysiol 93:283–295
Evola SV (1983) Chlorate-resistant variants of Nicotiana tabacum L. II. Parasexual genetic characterization. Mol Gen Genet 189:445–457
Glimelius K, Eriksson T, Grafe R, Müller AJ (1978) Somatic hybridisation of nitrate reductase-deficient mutants of Nicotiana tabacum by protoplast fusion. Physiol Plant 44:273–277
Grafe R, Müller AJ (1983) Complementation analysis of nitrate reductase deficient mutants of Nicotiana tabacum by somatic hybridisation. Theor Appl Genet 189:445
Jaworsky EG (1971) Nitrate reductase assay in intact plant tissues. Biochem Biophys Res Commun 43:1274–1279
Kleinhofs A, Warner RL, Warayan KR (1985) Current progress towards and understanding of the genetic and molecular biology of nitrate reductase in higher plants. Oxford Rev Plant Mol Biol Cell Biol 2, in press
Lazar GB, Frankhauser H, Potrykus I (1983) Complementation analysis of a nitrate reductase deficient Hyosciamus muticus cell line by somatic hybridisation. Mol Gen Genet 189:359–364
Marton L, Dung TM, Mendel RR, Maliga P (1982a) Nitrate reductase deficient cell lines from haploid protoplast cultures of Nicotiana plumbaginifolia. Mol Gen Genet 182:301–304
Marton L, Sidorov V, Biasini G, Maliga P (1982b) Complementation in somatic hybrids indicates four types of nitrate reductase deficient lines in Nicotiana plumbaginifolia. Mol Gen Genet 187:1–3
Menczel L, Nagy F, Kiss Z, Maliga P (1981) Streptomycine resistant and sensitive somatic hybrids of Nicotiana tabacum+Nicotiana knightiana: Correlation of resistance to N. tabacum plastids. Theor Appl Genet 59:191–195
Mendel RR, Alikulow ZA, Lvov NP, Müller AJ (1981) Presence of the molybdenum-cofactor in nitrate reductase-deficient mutant cell lines of Nicotiana tabacum. Mol Gen Genet 181:395–399
Müller AJ, Grafe R (1978) Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161:67–76
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assay with tobacco tissue culture. Physiol Plant 15:473–479
Negrutiu I (1981) Improved conditions for largescale culture, mutagenesis, and selection of haploid protoplasts of Nicotiana plumbaginifolia Viviani. Z Pflanzenphysiol 104:431–442
Negrutiu I, Dirks R, Jacobs M (1983) Regeneration of fully nitrate reductase-deficient mutants from protoplast culture of Nicotiana plumbaginifolia (Viviani). Theor Appl Genet 66:341–347
Negrutiu I, De Brouwer D, Famelaer I, Sidorov V, Jacobs M (1985) Use of biochemical mutants to study protoplast fusion parameters in Nicotiana plumbaginifolia. Plant Cell Reports In preparation
Steffen A, Schieder A (1984) Biochemical and genetical characterization of nitrate reductase deficient mutants of Petunia. Plant Cell Reports 3:134–137
Tomsett AB, Garrett RH (1979) The isolation and characterization of mutants defective in nitrate assimilation in Neurospora crassa. Genetics 95:649–660
Xuan LT, Grafe R, Müller AJ (1983) Complementation of nitrate reductase deficient mutants in somatic hybrids between nicotiana species. In: Protoplasts 1983. 6th International Protoplast Symposium Basel. Birkhäuser Verlag, Basel, p 75–76
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Dirks, R., Negrutiu, I., Sidorov, V. et al. Complementation analysis by somatic hybridisation and genetic crosses of nitrate reductase-deficient mutants of Nicotiana plumbaginifolia . Molec. Gen. Genet. 201, 339–343 (1985). https://doi.org/10.1007/BF00425681
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DOI: https://doi.org/10.1007/BF00425681