Summary
Six independently isolated mutants of Chlamydomonas reinhardtii that require elevated CO2 for photoautotrophic growth were tested by complementation analysis. These mutants are likely to be defective in some aspect of the algal concentrating mechanism for inorganic carbon as they exhibit CO2 fixation and inorganic carbon accumulation properties different from the wild-type. Four of the six mutants defined a single complementation group and appear to be defective in an intracellular carbonic anhydrase. The other two mutations represent two additional complementation groups.
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Abbreviations
- HS:
-
high salt medium which has 13 mM phosphate at pH 6.8
- HSA:
-
high salt plus 36 mM acetate medium
- YA:
-
high salt medium with 4 g yeast extract per L and 36mM acetate
- Arg:
-
arginine
- cia- :
-
CO2 accumulation mutants that cannot grow on low CO2
- Ci :
-
inorganic carbon (CO2+HCO 3- )
- CA:
-
carbonic anhydrase
- mt:
-
mating type
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Communicated by R.G. Herrmann
Supported in part by the McKnight Foundation and by NSF grant PCM 8005917 and published as journal article 11924 from the Michigan State Agriculatural Experiment Station
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Moroney, J.V., Tolbert, N.E. & Sears, B.B. Complementation analysis of the inorganic carbon concentrating mechanism of Chlamydomonas reinhardtii . Molec. Gen. Genet. 204, 199–203 (1986). https://doi.org/10.1007/BF00425498
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DOI: https://doi.org/10.1007/BF00425498